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anti-LIPF antibody :: Goat LIPF Polyclonal Antibody

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Catalog # MBS421137
Unit / Price
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  0.1 mg  /  $225 +1 FREE 8GB USB
anti-LIPF antibody
Product Name

LIPF, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-LIPF Antibody

Product Synonym Names
LIPF; lipase, gastric; GL; HGL; HLAL; MGC138477; MGC142271; gastric lipase; gastric triacylglycerol lipase
Antibody/Peptide Pairs
LIPF peptide (MBS425984) is used for blocking the activity of LIPF antibody (MBS421137)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
NDIVSMISED KK
OMIM
601980
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen
Peptide with sequence C-NDIVSMISEDKK, from the C Terminus of the protein sequence according to NP_004181.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-LIPF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-LIPF antibody
Peptide ELISA (EIA), Western Blot
Application Notes for anti-LIPF antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Preliminary experiments gave an approx 50kDa band in Human Duodenum lysates after 0. 02ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.2kDa according to NP_004181.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI/Uniprot data below describe general gene information for LIPF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
42,816 Da
NCBI Official Full Name
gastric triacylglycerol lipase isoform 2
NCBI Official Synonym Full Names
lipase F, gastric type
NCBI Official Symbol
NCBI Official Synonym Symbols
GL; HGL; HLAL
  [Similar Products]
NCBI Protein Information
gastric triacylglycerol lipase
UniProt Protein Name
Gastric triacylglycerol lipase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GL; Gastric lipase  [Similar Products]
UniProt Entry Name
LIPG_HUMAN
NCBI Summary for LIPF
This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
UniProt Comments for LIPF
LIPF: gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Protein type: EC 3.1.1.3; Hydrolase; Lipid Metabolism - glycerolipid; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q23.31

Molecular Function: triacylglycerol lipase activity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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