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anti-LRRFIP1 antibody :: Rabbit anti-Human LRRFIP1 Polyclonal Antibody

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Catalog # MBS151272
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

LRRFIP1, Polyclonal Antibody

Also Known As

LRRFIP1 Antibody

Product Synonym Names
LRRFIP1; GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1; GCF2; Leucine-rich repeat flightless-interacting protein 1; GC-binding factor 2; LRR FLII-interacting protein 1; leucine rich repeat (in FLII) interacting protein 1
Antibody/Peptide Pairs
LRRFIP1 peptide (MBS151970) is used for blocking the activity of LRRFIP1 antibody (MBS151272)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
808
OMIM
603256
3D Structure
ModBase 3D Structure for Q32MZ4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms.
Purity/Purification
LRRFIP1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
LRRFIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human LRRFIP1.
Buffer
LRRFIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
LRRFIP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-LRRFIP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-LRRFIP1 antibody
LRRFIP1 Antibody: LRRFIP1, also known as GC-binding factor 2 (GCF2), is a 738 amino acid transcriptional repressor that mainly plays a cytoskeletal role. It is primarily localized in the cytoplasm and preferentially binds to GC-rich dsDNA, but will also bind directly to dsRNA as well. The RNA binding domain encompasses a lysine-rich motif. LRRFIP1 interacts with the mammalian Flightless I (Fli-I) and is a key component in the cytoskeletal regulation of platelet function. LRRFIP1 and the related protein LRRFIP2 may modulate canonical WNT signaling and mediate the IRF3-induced production of type I interferon via a beta-catenin-dependent pathway.
Applications Tested/Suitable for anti-LRRFIP1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-LRRFIP1 antibody
LRRFIP1 antibody can be used for detection of LRRFIP1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-LRRFIP1 antibody
Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 μg/mL.
anti-LRRFIP1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-LRRFIP1 antibody
Immunofluorescence of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 20 μg/mL.
anti-LRRFIP1 antibody Immunofluorescence (IF) image
Immunohistochemistry (IHC) of anti-LRRFIP1 antibody
Immunohistochemistry of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 5 μg/mL.
anti-LRRFIP1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for LRRFIP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
73,060 Da
NCBI Official Full Name
leucine-rich repeat flightless-interacting protein 1 isoform 3
NCBI Official Synonym Full Names
leucine rich repeat (in FLII) interacting protein 1
NCBI Official Symbol
LRRFIP1  [Similar Products]
NCBI Official Synonym Symbols
GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1
  [Similar Products]
NCBI Protein Information
leucine-rich repeat flightless-interacting protein 1; GC-binding factor 2; NEDD8-conjugating enzyme; TAR RNA-interacting protein; LRR FLII-interacting protein 1
UniProt Protein Name
Leucine-rich repeat flightless-interacting protein 1
UniProt Synonym Protein Names
GC-binding factor 2; TAR RNA-interacting protein
UniProt Gene Name
LRRFIP1  [Similar Products]
UniProt Synonym Gene Names
GCF2; TRIP; LRR FLII-interacting protein 1  [Similar Products]
UniProt Entry Name
LRRF1_HUMAN
UniProt Comments for LRRFIP1
LRRFIP1: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. Belongs to the LRRFIP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; double-stranded RNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; negative regulation of transcription, DNA-dependent
Research Articles on LRRFIP1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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