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anti-SLC16A3 antibody :: Rabbit anti-Human MCT4 Polyclonal Antibody

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Catalog # MBS127546
Unit / Price
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Western Blot (WB)
Product Name

MCT4 (SLC16A3), Polyclonal Antibody

Popular Item
Full Product Name

MCT4 Polyclonal Antibody

Product Synonym Names
SLC16A3; MCT4; Monocarboxylate transporter 4
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
603877
3D Structure
ModBase 3D Structure for O15427
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Route
Synthetic Peptide
Category
Primary antibody
Immunogen
A synthetic peptide of human MCT4
Calculated Molecular Weight
49kDa
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-SLC16A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SLC16A3 antibody
Monocarboxylates, such as lactate and pyruvate, play an integral role in cellular metabolism. Lactic acid is produced in large quantities as a result of glycolysis, which provides the majority of ATP to cells under normal physiological conditions (2). However, accumulation of lactic acid leads to a decrease in intracellular pH and cessation of glycolysis (2). In order for glycolysis to continue at a high rate, lactic acid must be transported out of the cell (1,2). This transport process is carried out by a family of monocarboxylate transporters (MCTs), which function as proton symports and are stereoselective for L-lactate (1 -3).The MCT family consists of at least eight members, MCT 1-8, which contain between 10-12 transmembrane-helical (TM) domains, with the amino and carboxy termini located in the cytoplasm (4). MCT1 is widely expressed and is the major form of MCT in tumor cells and erythrocytes (5,6). MCT2 is highly expressed in liver and testis, while MCT3 and MCT4 are predominantly expressed in skeletal muscle (7,8).
Product Categories/Family for anti-SLC16A3 antibody
Applications Tested/Suitable for anti-SLC16A3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SLC16A3 antibody
WB: 1:200 - 1:500
IHC: 1:50 - 1:100

Western Blot (WB) of anti-SLC16A3 antibody
Western blot analysis of extracts of NCI-H460 cells, using SLC16A3 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
anti-SLC16A3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SLC16A3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Monocarboxylate transporter 4
NCBI Official Synonym Full Names
solute carrier family 16 (monocarboxylate transporter), member 3
NCBI Official Symbol
SLC16A3  [Similar Products]
NCBI Official Synonym Symbols
MCT3; MCT4; MCT 3; MCT 4; MCT-3; MCT-4
  [Similar Products]
NCBI Protein Information
monocarboxylate transporter 4; monocarboxylate transporter 3; solute carrier family 16 (monocarboxylic acid transporters), member 3; solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
UniProt Protein Name
Monocarboxylate transporter 4
UniProt Synonym Protein Names
Solute carrier family 16 member 3
UniProt Gene Name
SLC16A3  [Similar Products]
UniProt Synonym Gene Names
MCT4; MCT 4  [Similar Products]
UniProt Entry Name
MOT4_HUMAN
NCBI Summary for SLC16A3
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).[supplied by OMIM, Mar 2008]
UniProt Comments for SLC16A3
SLC16A3: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral; Transporter, SLC family

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: nuclear membrane; membrane; integral to plasma membrane; plasma membrane; actin cytoskeleton

Molecular Function: monocarboxylic acid transmembrane transporter activity; symporter activity; lactate transmembrane transporter activity; secondary active monocarboxylate transmembrane transporter activity

Biological Process: cellular metabolic process; monocarboxylic acid transport; pyruvate metabolic process; blood coagulation; leukocyte migration; transmembrane transport
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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