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anti-MGMT antibody :: Rabbit anti-Human Methylated-DNA--protein-cysteine methyltransferase Polyclonal Antibody

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Catalog # MBS7003994
Unit / Price
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  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

Methylated-DNA--protein-cysteine methyltransferase (MGMT), Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human Methylated-DNA--protein-cysteine methyltransferase polyclonal Antibody

Product Synonym Names
MGMT
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
156569
3D Structure
ModBase 3D Structure for P16455
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Immunogen
Recombinant human Methylated-DNA--protein-cysteine methyltransferase
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8825 / sc-8824 / sc-8826 / sc-28241
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-MGMT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MGMT antibody
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Applications Tested/Suitable for anti-MGMT antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-MGMT antibody
Recommended dilution: IHC: 1:20-1:200

Immunohistochemistry (IHC) of anti-MGMT antibody
Immunohistochemistry of paraffin-embedded human kidney using MBS7003994 at dilution of 1:20
anti-MGMT antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-MGMT antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS7003994 at dilution of 1:20
anti-MGMT antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for MGMT. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,646 Da[Similar Products]
NCBI Official Full Name
methylated-DNA--protein-cysteine methyltransferase isoform X2
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
UniProt Synonym Protein Names
6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
MGMT_HUMAN
NCBI Summary for MGMT
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
UniProt Comments for MGMT
MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.

Protein type: Apoptosis; EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 10q26

Cellular Component: membrane; nucleoplasm; nucleus

Molecular Function: calcium ion binding; damaged DNA binding; DNA binding; DNA-methyltransferase activity; methylated-DNA-[protein]-cysteine S-methyltransferase activity; methyltransferase activity

Biological Process: DNA dealkylation; DNA ligation; DNA methylation; DNA repair; positive regulation of DNA repair; regulation of caspase activity; response to drug; response to ethanol; response to folic acid; response to toxin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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