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anti-MAP1LC3a antibody :: Rabbit anti-Human Microtubule Associated Protein 1 Light Chain 3 Alpha Polyclonal Antibody

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Catalog # MBS2034049 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $115 +1 FREE 8GB USB
  0.02 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $190 +1 FREE 8GB USB
  0.1 mg  /  $245 +1 FREE 8GB USB
  0.2 mg  /  $370 +1 FREE 8GB USB
  1 mg  /  $880 +1 FREE 8GB USB
Product Name

Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a)

Product Synonym Names
MAP1ALC3; MAP1BLC3; LC3; LC3A; ATG8E; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF276658 mRNA
3D Structure
ModBase 3D Structure for Q9H492
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against MAP1LC3a. It has been selected for its ability to recognize MAP1LC3a in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Fragment
MAP1LC3a (Met1~Gly120)
Conjugate
No Conjugate
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant MAP1LC3a (Met1~Gly120) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2083185
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-MAP1LC3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-MAP1LC3a antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-MAP1LC3a antibody
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot (WB) of anti-MAP1LC3a antibody
Western Blot: Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Brain Tissue.
anti-MAP1LC3a antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-MAP1LC3a antibody
Western Blot: Sample: Recombinant MAP1LC3a, Human.
anti-MAP1LC3a antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for MAP1LC3a. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
14,493 Da
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3A isoform a
NCBI Official Synonym Full Names
microtubule associated protein 1 light chain 3 alpha
NCBI Official Symbol
MAP1LC3A  [Similar Products]
NCBI Official Synonym Symbols
LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
  [Similar Products]
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3A
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3A
UniProt Synonym Protein Names
Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
UniProt Gene Name
MAP1LC3A  [Similar Products]
UniProt Synonym Gene Names
MAP1A/MAP1B LC3 A  [Similar Products]
NCBI Summary for MAP1LC3a
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
UniProt Comments for MAP1LC3a
LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Microtubule-binding; Ubiquitin-like modifier; Vesicle

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: autophagic vacuole; cytosol; late endosome; microtubule

Molecular Function: phosphatidylethanolamine binding; phospholipid binding; protein binding; ubiquitin protein ligase binding

Biological Process: autophagosome assembly; cellular response to amino acid starvation; cellular response to nitrogen starvation; cellular response to starvation; macroautophagy; mitochondrion degradation; response to iron(II) ion; response to lead ion; response to morphine
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-MAP1LC3a antibody
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