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anti-MTHFD2 antibody :: Rabbit anti-Human MTHFD2 Polyclonal Antibody

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Catalog # MBS7044183
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

MTHFD2, Polyclonal Antibody

Popular Item
Full Product Name

MTHFD2 Antibody

Product Synonym Names
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; NAD-dependent methylenetetrahydrofolate dehydrogenase; MTHFD2; NMDMC
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
604887
3D Structure
ModBase 3D Structure for P13995
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Species
Human
Immunogen
Recombinant human Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial protein (40-250AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-74986 / sc-74985 / sc-74984
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-MTHFD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MTHFD2 antibody
extracellular space, mitochondrial matrix, mitochondrion, magnesium ion binding, methenyltetrahydrofolate cyclohydrolase activity, methylenetetrahydrofolate dehydrogenase (NAD+) activity, methylenetetrahydrofolate dehydrogenase (NADP+) activity, phosphate ion binding, folic acid metabolic process, one-carbon metabolic process
Applications Tested/Suitable for anti-MTHFD2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-MTHFD2 antibody
Western blot
All lanes: MTHFD2 antibody at 4.99 ug/ml
Lane 1: A431 whole cell lysate
Lane 2: HepG-2 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: Raji whole cell lysate
Lane 5: K562 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 38,27 kDa
Observed band size: 38,30,95 kDa

anti-MTHFD2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-MTHFD2 antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS7044183 at dilution of 1:100
anti-MTHFD2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-MTHFD2 antibody
Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7044183 at dilution of 1:100
anti-MTHFD2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for MTHFD2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
26,849 Da
NCBI Official Full Name
bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
NCBI Official Synonym Full Names
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
NCBI Official Symbol
MTHFD2  [Similar Products]
NCBI Official Synonym Symbols
NMDMC
  [Similar Products]
NCBI Protein Information
bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
UniProt Protein Name
Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
UniProt Gene Name
MTHFD2  [Similar Products]
UniProt Synonym Gene Names
NMDMC  [Similar Products]
UniProt Entry Name
MTDC_HUMAN
NCBI Summary for MTHFD2
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]
UniProt Comments for MTHFD2
MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Protein type: Cofactor and Vitamin Metabolism - one carbon pool by folate; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Hydrolase; EC 1.5.1.15; Mitochondrial; EC 3.5.4.9; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cytosol; extracellular space; mitochondrial matrix; mitochondrion

Molecular Function: formate-tetrahydrofolate ligase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; phosphate binding

Biological Process: folic acid metabolic process; one-carbon compound metabolic process; tetrahydrofolate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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