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anti-UNC13D antibody :: Goat Munc13-4/UNC13D Polyclonal Antibody

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Catalog # MBS420652
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Munc13-4/UNC13D, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-Munc13-4/UNC13D (C terminus) Antibody

Product Synonym Names
Goat Anti-Munc13-4 / UNC13D (Internal) Antibody; UNC13D; Munc13-4; unc-13 homolog D (C. elegans); FHL3; HLH3; HPLH3; unc-13 homolog D; UNC13D antibody; Munc13-4 antibody; unc-13 homolog D (C. elegans) antibody; FHL3 antibody; HLH3 antibody; HPLH3 antibody; unc-13 homolog D antibody; Munc13-4; Munc13-4 / UNC13D (C terminus)
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KQASQHALRP AP
OMIM
608897
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KQASQHALRPAP, from the C Terminus of the protein sequence according to NP_954712.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-UNC13D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-UNC13D antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-UNC13D antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx.105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-UNC13D antibody
(2ug/ml) staining of Human T-lymphocyte lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Data provided by anonymous customer.
anti-UNC13D antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for UNC13D. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
128,819 Da
NCBI Official Full Name
protein unc-13 homolog D
NCBI Official Synonym Full Names
unc-13 homolog D
NCBI Official Symbol
UNC13D  [Similar Products]
NCBI Official Synonym Symbols
FHL3; HLH3; HPLH3; Munc13-4
  [Similar Products]
NCBI Protein Information
protein unc-13 homolog D
UniProt Protein Name
Protein unc-13 homolog D
UniProt Synonym Protein Names
Munc13-4
Protein Family
UniProt Gene Name
UNC13D  [Similar Products]
UniProt Entry Name
UN13D_HUMAN
NCBI Summary for UNC13D
This gene encodes a protein that is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis type 3, a genetically heterogeneous, rare autosomal recessive disorder. [provided by RefSeq, Jul 2008]
UniProt Comments for UNC13D
UNC13D: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells. Defects in UNC13D are the cause of familial hemophagocytic lymphohistiocytosis type 3 (FHL3); also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T-lymphocytes in lymph nodes, spleen, and other organs is also found. Belongs to the unc-13 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: late endosome; lysosome; membrane; recycling endosome

Molecular Function: protein binding; Rab GTPase binding

Biological Process: defense response to virus; germinal center formation; granuloma formation; natural killer cell degranulation; phagocytosis; positive regulation of exocytosis; regulation of mast cell degranulation

Disease: Hemophagocytic Lymphohistiocytosis, Familial, 3
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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