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anti-Slc11a1 antibody :: Rabbit anti-Human Natural Resistance Associated Macrophage Protein Polyclonal Antibody

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Catalog # MBS612130
Unit / Price
  0.1 mL  /  $720 +1 FREE 8GB USB
anti-Slc11a1 antibody
Product Name

Natural Resistance Associated Macrophage Protein (Slc11a1), Polyclonal Antibody

Full Product Name

Natural Resistance Associated Macrophage Protein (Nramp2)

Product Synonym Names
Anti -Natural Resistance Associated Macrophage Protein (Nramp2)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000067.5 (74421777..74432625). Location: 1 C3; 1 39.2 cM
3D Structure
ModBase 3D Structure for P41251
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The 17 AA human control peptide sequence is 88% conserved in monkey NRAMP2 with IRE. This sequence is absent in NRAMP2 without IRE. No significant sequence homology exist with NRAMP1.
Purity/Purification
Serum
Serum
Form/Format
Supplied as a liquid, 0.05% sodium azide, 40% glycerol.
Immunogen
A 17 AA Peptide sequence located at the predicted cytoplasmic loop near the C-terminus of human NRAMP2, with IRE (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-Slc11a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Slc11a1 antibody
Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has recently been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgarde (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.
Applications Tested/Suitable for anti-Slc11a1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Slc11a1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:5000
ELISA: 1:10,000-50,000
NCBI/Uniprot data below describe general gene information for Slc11a1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,741 Da[Similar Products]
NCBI Official Full Name
natural resistance associated macrophage protein
NCBI Official Synonym Full Names
solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
NCBI Official Symbol
Slc11a1  [Similar Products]
NCBI Official Synonym Symbols
Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157
  [Similar Products]
NCBI Protein Information
natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh
UniProt Protein Name
Natural resistance-associated macrophage protein 1
UniProt Gene Name
Slc11a1  [Similar Products]
UniProt Synonym Gene Names
Bcg; Ity; Lsh; Nramp1  [Similar Products]
UniProt Entry Name
NRAM1_MOUSE
UniProt Comments for Slc11a1
SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Transporter, SLC family; Membrane protein, integral; Membrane protein, multi-pass; Vesicle; Transporter; Cell surface

Cellular Component: phagocytic vesicle membrane; cell surface; membrane; lysosome; late endosome; plasma membrane; integral to membrane; endosome membrane; vacuole

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; transporter activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; positive regulation of T-helper 1 type immune response; wound healing; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; antigen processing and presentation of peptide antigen; vacuolar acidification; nitrite transport; transport; T cell proliferation during immune response; T cell cytokine production; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; positive regulation of cytokine production; mRNA stabilization; MAPKKK cascade; L-arginine import; defense response to protozoan; interleukin-2 production; iron ion transport; phagocytosis; cellular cadmium ion homeostasis; MHC class II biosynthetic process; positive regulation of interferon-gamma production; iron ion homeostasis; macrophage activation; response to bacterium; protein import into nucleus, translocation; negative regulation of cytokine production; defense response to bacterium; activation of protein kinase activity; ion transport; positive regulation of transcription from RNA polymerase II promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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