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anti-NELFA antibody :: Mouse anti-Human NELFA Polyclonal Antibody

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Catalog # MBS6001042
Unit / Price
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  0.05 mg  /  $580 +1 FREE 8GB USB
Product Name

NELFA, Polyclonal Antibody

Popular Item
Full Product Name

NELFA (Negative Elongation Factor A, NELF-A, Wolf-Hirschhorn Syndrome Candidate 2 Protein, WHSC2, P/OKcl.15)

Product Synonym Names
Anti -NELFA (Negative Elongation Factor A, NELF-A, Wolf-Hirschhorn Syndrome Candidate 2 Protein, WHSC2, P/OKcl.15)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT LHLPRRTVDE MKGALMEIIQ LASLDSDPWV LMVADILKSF PDTGSLNLEL EEQNPNVQDI LGELREKVGE CEASAMLPLE CQYLNKNALT TLAGPLTPPV KHFQLKRKPK SATLRAELLQ KSTETAQQLK RSAGVPFHAK GRGLLRKMDT TTPLKGIPKQ APFRSPTAPS VFSPTGNRTP IPPSRTLLRK ERGVKLLDIS ELDMVGAGRE AKRRRKTLDA EVVEKPAKEE TVVENATPDY AAGLVSTQKL GSLNNEPALP STSYLPSTPS VVPASSYIPS SETPPAPSSR EASRPPEEPS APSPTLPAQF KQRAPMYNSG LSPATPTPAA PTSPLTPTTP PAVAPTTQTP PVAMVAPQTQ APAQQQPKKN LSLTREQMFA AQEMFKTANK VTRPEKALIL GFMAGSRENP CQEQGDVIQI KLSEHTEDLP KADGQGSTTM LVDTVFEMNY ATGQWTRFKK YKPMTNVS
Chromosome Location
Chromosome: 4; NC_000004.11 (1984441..2010962, complement). Location: 4p16.3
OMIM
606026
3D Structure
ModBase 3D Structure for Q9H3P2
Clonality
Polyclonal
Isotype
IgG
Host
Mouse
Species Reactivity
Human
Specificity
Recognizes human WHSC2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Concentration
1mg/ml (lot specific)
Immunogen
Full length human WHSC2, aa1-528 (NP_005654.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Small volumes of anti-NELFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-NELFA antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-NELFA antibody
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml

Western Blot of anti-NELFA antibody
WHSC2 polyclonal antibody Western Blot analysis of WHSC2 expression in Jurkat.
anti-NELFA antibody Western Blot (WB) image
Western Blot of anti-NELFA antibody
Western Blot analysis of WHSC2 expression in transfected 293T cell line by WHSC2 polyclonal antibody. Lane 1: WHSC2 transfected lysate (58.08kD). Lane 2: Non-transfected lysate.
anti-NELFA antibody Western Blot (WB) image
Immunofluorescence of anti-NELFA antibody
Immunofluorescence of purified antibody to WHSC2 on HeLa cell. (Concentration 10ug/ml).
anti-NELFA antibody Immunofluorescence image
NCBI/Uniprot data below describe general gene information for NELFA. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,277 Da[Similar Products]
NCBI Official Full Name
Negative elongation factor A
NCBI Official Synonym Full Names
negative elongation factor complex member A
NCBI Official Symbol
NELFA  [Similar Products]
NCBI Official Synonym Symbols
WHSC2; NELF-A; P/OKcl.15
  [Similar Products]
NCBI Protein Information
negative elongation factor A; wolf-Hirschhorn syndrome candidate 2 protein
UniProt Protein Name
Negative elongation factor A
UniProt Synonym Protein Names
Wolf-Hirschhorn syndrome candidate 2 protein
UniProt Gene Name
NELFA  [Similar Products]
UniProt Synonym Gene Names
WHSC2; NELF-A  [Similar Products]
UniProt Entry Name
NELFA_HUMAN
NCBI Summary for NELFA
This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]
UniProt Comments for NELFA
WHSC2: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. Belongs to the NELF-A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; multicellular organismal development; RNA elongation from RNA polymerase II promoter; gene expression
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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