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anti-NIX antibody :: Rabbit anti-Human NIX / BNIP3L Polyclonal Antibody

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Catalog # MBS240473
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

NIX / BNIP3L, Polyclonal Antibody

Also Known As

Rabbit Polyclonal to Human NIX / BNIP3L

Product Synonym Names
Anti-NIX / BNIP3L Antibody (aa77-92) IHC-plus; BNIP3L; NIP3-like protein X; NIP3L; NIX; BNIP3a; BNIP3H; Human NIX; BNIP3L
Product Gene Name
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
219
OMIM
605368
3D Structure
ModBase 3D Structure for O60238
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
peptide (daqhesgqsssrgssh) corresponding to amino acids 77 to 92 of human origin, which are identical to those of mouse Bnip3L
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide.
Concentration
0.5 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human BNIP3L.
Immunogen Type
Synthetic peptide
Immunogen
NIX / BNIP3L antibody was raised against synthetic peptide from human BNIP3L.
Antigen Modification
aa77-92
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Other Notes
Small volumes of anti-NIX antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NIX antibody
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X).
Product Categories/Family for anti-NIX antibody
Applications Tested/Suitable for anti-NIX antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-NIX antibody
IHC-P (5 ug/ml), WB (0.5 - 1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-NIX antibody
Anti-BNIP3L antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-NIX antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-NIX antibody
Immunofluorescence of Bnip3L in Human Kidney cells with Bnip3L antibody at 10 ug/ml.
anti-NIX antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-NIX antibody
Western blot of Bnip3L in K562 whole cell lysate in the absence (A), or presence (B) of blocking peptide with Bnip3L antibody (IN) at 1 ug/ml.
anti-NIX antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NIX. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
19,559 Da
NCBI Official Full Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
NCBI Official Synonym Full Names
BCL2/adenovirus E1B 19kDa interacting protein 3-like
NCBI Official Symbol
BNIP3L  [Similar Products]
NCBI Official Synonym Symbols
NIX; BNIP3a
  [Similar Products]
NCBI Protein Information
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; NIP3L; NIP3-like protein X; adenovirus E1B19k-binding protein B5; BCL2/adenovirus E1B 19-kd protein-interacting protein 3a; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A
UniProt Protein Name
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
UniProt Synonym Protein Names
Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIP3-like protein X; NIP3L
UniProt Gene Name
BNIP3L  [Similar Products]
UniProt Synonym Gene Names
BNIP3A; BNIP3H; NIX; NIP3L  [Similar Products]
UniProt Entry Name
BNI3L_HUMAN
NCBI Summary for NIX
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein counteracts the apoptotic inducer BNIP3 and may play a role in tumor suppression. [provided by RefSeq, Mar 2011]
UniProt Comments for NIX
BNIP3L: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. May function as a tumor suppressor. Self-associates. Interacts with BNIP3 and STEAP3. Interacts with SPATA18/MIEAP. Interacts with human adenovirus-2 E1B 19 kDa protein. Belongs to the NIP3 family.

Protein type: Apoptosis; Membrane protein, integral; Endoplasmic reticulum; Nuclear envelope; Mitochondrial

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; integral to membrane; intrinsic to membrane; nuclear envelope

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; lamin binding

Biological Process: viral reproduction; positive regulation of apoptosis; defense response to virus; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Pathways associated with anti-NIX antibody
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