• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-NOX5 antibody :: Rabbit anti-Human NOX5 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS176174
Unit / Price
  0.1 mg  /  $315 +1 FREE 8GB USB
Product Name

NOX5, Polyclonal Antibody

Popular Item
Full Product Name

Anti-NOX5 antibody

Product Synonym Names
NADPH oxidase 5; NADPH oxidase, EF-hand calcium binding domain 5; MGC149776 antibody; MGC149777 antibody; NADPH oxidase 5 antibody; NADPH oxidase EFhand calcium binding domain 5 antibody; NOX5 antibody; NOX5_HUMAN antibody
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
606572
3D Structure
ModBase 3D Structure for Q96PH1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5 (747-765aa KVLKGHCEKFGFRFFQENF).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-NOX5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NOX5 antibody
Description: Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.
Background: NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.
Applications Tested/Suitable for anti-NOX5 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC)
Application Notes for anti-NOX5 antibody
Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Hu
IHC (Paraffin-embedded section): Concentration: 0.5-1ug/ml; Tested Species: Hu; Antigen Retrieval: By Heat.
ICC: Concentration: 0.5-1ug/ml; Tested Species: Hu
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections to 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.

Immunohistochemistry (IHC) of anti-NOX5 antibody
Anti-NOX5 antibody, MBS176174, IHC(P)
IHC(P): Human Mammary Cancer Tissue
anti-NOX5 antibody Immunohistochemistry (IHC) (IHC) image
Immunocytochemistry (ICC) of anti-NOX5 antibody
Anti-NOX5 antibody, MBS176174, ICC
ICC: HELA Cell
anti-NOX5 antibody Immunocytochemistry (ICC) (ICC) image
Western Blot (WB) of anti-NOX5 antibody
Anti-NOX5 antibody, MBS176174, Western blotting
All lanes: Anti (MBS176174) at 0.5ug/ml
Lane 1: 22RV1 Whole Cell Lysate at 40ug
Lane 2: PANC Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 86KD
Observed bind size: 86KD
anti-NOX5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for NOX5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
83,109 Da
NCBI Official Full Name
NADPH oxidase 5
NCBI Official Synonym Full Names
NADPH oxidase, EF-hand calcium binding domain 5
NCBI Official Symbol
NCBI Protein Information
NADPH oxidase 5
UniProt Protein Name
NADPH oxidase 5
Protein Family
UniProt Gene Name
UniProt Entry Name
NOX5_HUMAN
NCBI Summary for NOX5
This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for NOX5
NOX5: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.6.3.-; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: endoplasmic reticulum; integral to membrane

Molecular Function: protein binding; FAD binding; superoxide-generating NADPH oxidase activity; heme binding; calcium ion binding; hydrogen ion channel activity; NADP binding

Biological Process: cell proliferation; proton transport; regulation of fusion of sperm to egg plasma membrane; apoptosis; endothelial cell proliferation; cytokinesis; regulation of proton transport; angiogenesis; cytokine secretion; superoxide release
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000