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anti-NR0B1 antibody :: Rabbit anti-Human NR0B1 Polyclonal Antibody

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Catalog # MBS7052197
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

NR0B1, Polyclonal Antibody

Popular Item
Also Known As

NR0B1 Antibody

Product Synonym Names
Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1; NR0B1; AHC; DAX1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
470
OMIM
S74720 mRNA
3D Structure
ModBase 3D Structure for P51843
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human Nuclear receptor subfamily 0 group B member 1 protein (1-243AA)
Conjugation
Non-conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-NR0B1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NR0B1 antibody
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
Applications Tested/Suitable for anti-NR0B1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NR0B1 antibody
WB: 1:500-1:5000
IHC: 1:20-1:200

Western Blot (WB) of anti-NR0B1 antibody
Western Blot
Positive WB detected in: MCF-7 whole cell lysate,Hela whole cell lysate
All lanes: NR0B1 antibody at 2.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52,44 KDa
Observed band size: 52 KDa

anti-NR0B1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-NR0B1 antibody
Immunohistochemistry of paraffin-embedded human testis tissue using MBS7052197 at dilution of 1:100
anti-NR0B1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-NR0B1 antibody
Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7052197 at dilution of 1:100
anti-NR0B1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for NR0B1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
43,590 Da
NCBI Official Full Name
nuclear receptor subfamily 0 group B member 1
NCBI Official Synonym Full Names
nuclear receptor subfamily 0 group B member 1
NCBI Official Symbol
NR0B1  [Similar Products]
NCBI Official Synonym Symbols
AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
  [Similar Products]
NCBI Protein Information
nuclear receptor subfamily 0 group B member 1
UniProt Protein Name
Nuclear receptor subfamily 0 group B member 1
UniProt Synonym Protein Names
DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1
UniProt Gene Name
NR0B1  [Similar Products]
UniProt Synonym Gene Names
AHC; DAX1  [Similar Products]
NCBI Summary for NR0B1
This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq, Jul 2008]
UniProt Comments for NR0B1
NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: Xp21.2

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus; polysomal ribosome

Molecular Function: DNA binding; DNA hairpin binding; ligand-dependent nuclear receptor activity; protein binding; protein domain specific binding; protein homodimerization activity; RNA binding; sequence-specific DNA binding; steroid hormone receptor binding; transcription corepressor activity; transcription factor binding

Biological Process: adrenal gland development; gonad development; male gonad development; negative regulation of steroid hormone receptor signaling pathway; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; protein localization; steroid biosynthetic process; transcription initiation from RNA polymerase II promoter

Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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