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anti-SIRT1 antibody :: Goat anti-Human Nuclear Receptor LXR alpha, beta Polyclonal Antibody

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Catalog # MBS619896
Unit / Price
  0.1 mg  /  $755 +1 FREE 8GB USB
anti-SIRT1 antibody
Product Name

Nuclear Receptor LXR alpha, beta (SIRT1), Polyclonal Antibody

Full Product Name

Nuclear Receptor LXR alpha, beta (Liver X Receptor alpha, Liver X Receptor beta, LX Receptor alpha, LX Receptor beta, LXRa, LXR-a, LXRalpha, LXRb, LXR-b, LXRbeta, NERI, NER-I, NR1H2, NR1H3, Nuclear Receptor NER, Nuclear Receptor Subfamily 1 Group H Member

Product Synonym Names
Anti -Nuclear Receptor LXR alpha, beta (Liver X Receptor alpha, Liver X Receptor beta, LX Receptor alpha, LX Receptor beta, LXRa, LXR-a, LXRalpha, LXRb, LXR-b, LXRbeta, NERI, NER-I, NR1H2, NR1H3, Nuclear Receptor NER, Nuclear Receptor Subfamily 1 Group H Member
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 10; NC_000010.10 (69644427..69678147). Location: 10q21.3
OMIM
604479
3D Structure
ModBase 3D Structure for Q96EB6
Clonality
Polyclonal
Isotype
IgG
Host
Goat
Species Reactivity
Human
Specificity
Recognizes an epitope corresponding to 429-442aa of human LXR (liver X receptor) alpha and to 443-456aa of human LXR beta. Species sequence homology: mouse and rat.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in tris-saline, pH 7.3, 0.02% sodium azide, 0.5%BSA.
Immunogen
Peptide sequence CRLQDKKLPPLLSEI corresponding to the internal region of LXR Alpha/Beta (NP_005684; NP_009052).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-SIRT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SIRT1 antibody
LXR alpha and beta are members of the nuclear hormone receptor superfamily of ligand-activated transcription factors, and each have a molecular weight of 55kD.
Product Categories/Family for anti-SIRT1 antibody
Applications Tested/Suitable for anti-SIRT1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-SIRT1 antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32,000
Western Blot: 1-3ug/ml
NCBI/Uniprot data below describe general gene information for SIRT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
81,681 Da[Similar Products]
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-1
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1  [Similar Products]
NCBI Official Synonym Symbols
SIR2L1
  [Similar Products]
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Synonym Protein Names
Regulatory protein SIR2 homolog 1; SIR2-like protein 1
UniProt Gene Name
SIRT1  [Similar Products]
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1  [Similar Products]
UniProt Entry Name
SIR1_HUMAN
NCBI Summary for SIRT1
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
UniProt Comments for SIRT1
SIRT1: an NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separate cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. Elevation of NAD(+)/NADP(+) ratio activates SIRT1. Recruited to LRH1 target gene promoters by NR0B2/SHP thereby stimulating histone H3 and H4 deacetylation leading to transcriptional repression. Implicated in regulation of adipogenesis and fat mobilization in white adipocytes by repression of PPARG. Involved in liver and muscle metabolism. Is involved in autophagy, presumably by deacetylating ATG5, ATG7 and ATG8. Deacetylates AKT1 which leads to enhanced binding of AKT1 and PDK1 to PIP3 and promotes their activation. Widely expressed. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Deacetylase; EC 3.5.1.-; Apoptosis

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: nucleoplasm; chromatin silencing complex; PML body; mitochondrion; ESC/E(Z) complex; nuclear chromatin; cytoplasm; nucleolus; nuclear inner membrane; nuclear envelope; nuclear heterochromatin; nucleus

Molecular Function: protein C-terminus binding; deacetylase activity; identical protein binding; histone binding; p53 binding; metal ion binding; protein deacetylase activity; transcription factor binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; enzyme binding; NAD-dependent histone deacetylase activity; bHLH transcription factor binding; mitogen-activated protein kinase binding; histone deacetylase activity; HLH domain binding; transcription corepressor activity; NAD+ ADP-ribosyltransferase activity

Biological Process: muscle development; establishment and/or maintenance of chromatin architecture; viral reproduction; positive regulation of apoptosis; regulation of mitotic cell cycle; protein ubiquitination; positive regulation of caspase activity; negative regulation of prostaglandin biosynthetic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of gene expression, epigenetic; behavioral response to starvation; positive regulation of adaptive immune response; positive regulation of histone H3-K9 methylation; positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of transcription factor activity; pyrimidine dimer repair via nucleotide-excision repair; cell aging; negative regulation of I-kappaB kinase/NF-kappaB cascade; cellular response to starvation; negative regulation of fat cell differentiation; ovulation from ovarian follicle; cholesterol homeostasis; regulation of endodeoxyribonuclease activity; protein amino acid ADP-ribosylation; methylation-dependent chromatin silencing; inhibition of NF-kappaB transcription factor; maintenance of chromatin silencing; negative regulation of phosphorylation; regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; triacylglycerol mobilization; negative regulation of transcription, DNA-dependent; rRNA processing; peptidyl-lysine acetylation; negative regulation of apoptosis; chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; establishment of chromatin silencing; chromatin silencing; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; response to insulin stimulus; DNA synthesis during DNA repair; protein amino acid deacetylation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; circadian regulation of gene expression; angiogenesis; DNA replication; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; single strand break repair; positive regulation of chromatin silencing; negative regulation of TOR signaling pathway; negative regulation of protein kinase B signaling cascade; protein destabilization; histone deacetylation; DNA repair; regulation of cell proliferation; negative regulation of helicase activity; response to hydrogen peroxide; fatty acid homeostasis; white fat cell differentiation; cell glucose homeostasis; spermatogenesis; gene expression; positive regulation of insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of cell growth; negative regulation of transforming growth factor beta receptor signaling pathway; response to DNA damage stimulus; positive regulation of macroautophagy
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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