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anti-SQSTM1 antibody :: Rabbit anti-Human p62 (Ser28) Polyclonal Antibody

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Catalog # MBS502205
Unit / Price
  0.1 mL  /  $360 +1 FREE 8GB USB
Testing Data
Product Name

p62 (Ser28) (SQSTM1), Polyclonal Antibody

Full Product Name

Anti-Phospho- Ser28 p62

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 5; NC_000005.10 (179806388..179838078). Location: 5q35
OMIM
601530
3D Structure
ModBase 3D Structure for Q13501
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
Specific for the ~48k p62 protein phosphorylated at Ser28.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser28 of p62.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser28 conjugated to KLH
Immunogen Species
Human
Reactivity Assumed Based on 100% Sequence Homology
Non-human primates
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with human tissue. It is anticipated that the antibody will also react with non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
p62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease (Nogalaska et al., 2009). p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages (Nakaso et al., 2004). Phosphorylation of Ser28 has recently been demonstrated to be related to the pathogenesis of Parkinson's disease.
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Other Notes
Small volumes of anti-SQSTM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SQSTM1 antibody
Affinity purified rabbit polyclonal antibody
Applications Tested/Suitable for anti-SQSTM1 antibody
Western Blot (WB)
Application Notes for anti-SQSTM1 antibody
Quality Control: Western bots performed on each lot.
WB: 1:1000

Testing Data of anti-SQSTM1 antibody
anti-SQSTM1 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for SQSTM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48
NCBI Official Full Name
sequestosome-1 isoform 2
NCBI Official Synonym Full Names
sequestosome 1
NCBI Official Symbol
SQSTM1  [Similar Products]
NCBI Official Synonym Symbols
p60; p62; A170; OSIL; PDB3; ZIP3; p62B
  [Similar Products]
NCBI Protein Information
sequestosome-1; EBIAP; EBI3-associated protein p60; oxidative stress induced like; ubiquitin-binding protein p62; EBI3-associated protein of 60 kDa; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
UniProt Protein Name
Sequestosome-1
UniProt Synonym Protein Names
EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62
Protein Family
UniProt Gene Name
SQSTM1  [Similar Products]
UniProt Synonym Gene Names
ORCA; OSIL; EBIAP; p60  [Similar Products]
UniProt Entry Name
SQSTM_HUMAN
NCBI Summary for SQSTM1
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
UniProt Comments for SQSTM1
Function: Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures). Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD

By similarity. May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Ref.12 Ref.14 Ref.15 Ref.19 Ref.24 Ref.25 Ref.26 Ref.27 Ref.29 Ref.31 Ref.53

Subunit structure: Homooligomer or heterooligomer; may form homotypic arrays. Dimerization interferes with ubiquitin binding. Interacts directly with PRKCI and PRKCZ

Probable. Forms ternary complexes with PRKCZ and KCNAB2 or PRKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6

By similarity. Interacts with EBI3, LCK, RASA1, PRKCZ, PRKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MAP2K5, TRIM13, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63-polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MAP2K5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. Interacts with TRAF6 and CYLD. Identified in a complex with TRAF6 and CYLD

By similarity. Identified in a heterotrimeric complex with ubiquitin and ZFAND5, where ZFAND5 and SQSTM1 both interact with the same ubiquitin molecule. Directly interacts with MAP1LC3A and MAP1LC3B, as well as with other MAP1 LC3 family members, including GABARAP, GABARAPL1 and GABARAPL2; these interactions are necessary for the recruitment MAP1 LC3 family members to inclusion bodies containing polyubiquitinated protein aggregates and for their degradation by autophagy. Interacts with FHOD3. Interacts with TRMI5. Ref.1 Ref.2 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.17 Ref.19 Ref.20 Ref.21 Ref.24 Ref.26 Ref.27 Ref.28 Ref.31 Ref.34 Ref.40 Ref.41 Ref.44 Ref.46 Ref.47 Ref.49 Ref.51 Ref.53

Subcellular location: Cytoplasm. Late endosome. Lysosome. Cytoplasmic vesicle › autophagosome. Nucleus. Endoplasmic reticulum. Cytoplasm › P-body. Note: Sarcomere

By similarity. In cardiac muscles localizes to the sarcomeric band

By similarity. Commonly found in inclusion bodies containing polyubiquitinated protein aggregates. In neurodegenerative diseases, detected in Lewy bodies in Parkinson disease, neurofibrillary tangles in Alzheimer disease, and HTT aggregates in Huntington disease. In protein aggregate diseases of the liver, found in large amounts in Mallory bodies of alcoholic and nonalcoholic steatohepatitis, hyaline bodies in hepatocellular carcinoma, and in SERPINA1 aggregates. Enriched in Rosenthal fibers of pilocytic astrocytoma. In the cytoplasm, observed in both membrane-free ubiquitin-containing protein aggregates (sequestosomes) and membrane-surrounded autophagosomes. Colocalizes with TRIM13 in the perinuclear endoplasmic reticulum. Co-localizes with TRIM5 in the cytoplasmic bodies. Ref.7 Ref.11 Ref.13 Ref.16 Ref.18 Ref.19 Ref.23 Ref.26 Ref.27 Ref.41 Ref.44 Ref.46

Tissue specificity: Ubiquitously expressed. Ref.2

Induction: By proteasomal inhibitor PSI and prostaglandin J2 (PGJ2) (at protein level). By phorbol 12-myristate 13-acetate (PMA). Ref.6 Ref.22 Ref.29

Domain: The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55. Both the UBA and OPR domains are necessary and sufficient for the localization into the ubiquitin-containing inclusion bodies. Ref.14 Ref.20 Ref.21 Ref.24 Ref.26 Ref.27 Ref.31 Ref.50The OPR domain mediates homooligomerization and interactions with FHOD3, MAP2K5, NBR1, PRKCI and PRKCZ. Both the OPR and UBA domains are necessary and sufficient for the localization into the ubiquitin-containing inclusion bodies. Ref.14 Ref.20 Ref.21 Ref.24 Ref.26 Ref.27 Ref.31 Ref.50The ZZ-type zinc finger mediates the interaction with RIPK1. Ref.14 Ref.20 Ref.21 Ref.24 Ref.26 Ref.27 Ref.31 Ref.50

Post-translational modification: Phosphorylated. May be phosphorylated by PRKCZ

By similarity. Phosphorylated in vitro by TTN. Ref.31

Involvement in disease: Paget disease of bone (PDB) [MIM:602080]: Metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.53 Ref.54 Ref.55 Ref.56 Ref.57 Ref.58 Ref.59 Ref.60In a cell model for Huntington disease (HD), appears to form a shell surrounding aggregates of mutant HTT that may protect cells from apoptosis, possibly by recruiting autophagosomal components to the polyubiquitinylated protein aggregates. Ref.26

Sequence similarities: Contains 1 OPR domain.Contains 1 UBA domain.Contains 1 ZZ-type zinc finger.
Product References and Citations for anti-SQSTM1 antibody
• Nogalska A, Terracciano C, D'Agostino C, Engel WK, Askanas V (2009). p62/SQSTM1 is overexpressed and prominently accumulated in inclusions of sporadic inclusion-body myositis muscle fibers, and can help differentiating it from polymyositis and dermatomyositis. Acta Neuropathol. 3: 407-13.
• Nakaso K, Yoshimoto Y, Nakano T, Takeshima T, Fukuhara Y, Yasui K, Araga S, Yanagawa T, Ishii T, Nakashima K. (2004). Transcriptional activation of p62/A170/ZIP during the formation of the aggregates: possible mechanisms and the role in Lewy body formation in Parkinson's disease. Brain Res. 1-2: 42-51.

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