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anti-PAF1 antibody :: Rabbit anti-Human PAF1 Polyclonal Antibody

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Catalog # MBS7002771
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

PAF1, Polyclonal Antibody

Popular Item
Also Known As

PAF1 Antibody

Product Synonym Names
RNA polymerase II-associated factor 1 homolog; hPAF1; Pancreatic differentiation protein 2; PAF1; PD2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
485
OMIM
610506
3D Structure
ModBase 3D Structure for Q8N7H5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%,Protein G purified
Form/Format
Liquid
Immunogen
Recombinant human RNA polymerase II-associated factor 1 homolog protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PAF1 antibody
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro.
Applications Tested/Suitable for anti-PAF1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-PAF1 antibody
Western Blot
Positive WB detected in:HepG2 whole cell lysate,Hela whole cell lysate,K562 whole cell lysate,Mouse brain tissue
All lanes: PAF1 antibody at 3.2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60,56 kDa
Observed band size: 60,48 kDa

anti-PAF1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PAF1 antibody
Immunohistochemistry of paraffin-embedded human testicular tissue using MBS7002771 at dilution of 1:100
anti-PAF1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PAF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
55,473 Da
NCBI Official Full Name
RNA polymerase II-associated factor 1 homolog isoform 2
NCBI Official Synonym Full Names
PAF1 homolog, Paf1/RNA polymerase II complex component
NCBI Official Symbol
NCBI Official Synonym Symbols
PD2; F23149_1
  [Similar Products]
NCBI Protein Information
RNA polymerase II-associated factor 1 homolog
UniProt Protein Name
RNA polymerase II-associated factor 1 homolog
UniProt Synonym Protein Names
Pancreatic differentiation protein 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PD2; hPAF1  [Similar Products]
UniProt Entry Name
PAF1_HUMAN
NCBI Summary for PAF1
This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]
UniProt Comments for PAF1
PAF1: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the RNF20/40 E3 ubiquitin-protein ligase complex. Involved in polyadenylation of mRNA precursors. Has oncogenic activity in vivo and in vitro. Belongs to the PAF1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Tumor suppressor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell junction; cytoplasm; membrane; nucleoplasm

Molecular Function: chromatin binding; protein binding

Biological Process: endodermal cell fate commitment; histone H2B ubiquitination; histone monoubiquitination; mRNA polyadenylation; negative regulation of myeloid cell differentiation; negative regulation of transcription from RNA polymerase II promoter; nucleosome positioning; positive regulation of histone methylation; positive regulation of mRNA 3'-end processing; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; RNA elongation from RNA polymerase II promoter; stem cell maintenance; Wnt receptor signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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