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anti-PCK1 antibody :: Goat PCK1/PEPCKC Polyclonal Antibody

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Catalog # MBS420472
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

PCK1/PEPCKC, Polyclonal Antibody

Also Known As

Goat anti-PCK1/PEPCKC (internal region) Antibody

Product Synonym Names
Goat Anti-PCK1 / PEPCKC (internal) Antibody; PCK1; PEPCKC; phosphoenolpyruvate carboxykinase 1 (soluble); MGC22652; PEPCK1; PEP carboxykinase; cytosolic phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; PCK1 antibody; PEPCKC antibody; phosphoenolpyruvate carboxykinase 1 (soluble) antibody; MGC22652 antibody; PEPCK1 antibody; PEP carboxykinase antibody; cytosolic phosphoenolpyruvate carboxykinase 1 antibody; phosphoenolpyruvate carboxylase antibody; phosphopyruvate carboxylase antibody; PCK1 / PEPCKC (internal region)
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
622
Immunogen Sequence
EKEVEDIEKY LEDQ
OMIM
261680
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-EKEVEDIEKYLEDQ, from the internal region (near the C Terminus) of the protein sequence according to NP_002582.2.
Epitope
Internal region (near the C Terminus)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-PCK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PCK1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PCK1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 70kDa band observed in Human Kidney lysates (calculated MW of 69.2kDa according to NP_002582.2). Recommended concentration: 0.5-1.5ug/ml.

Western Blot (WB) of anti-PCK1 antibody
(0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PCK1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PCK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,156 Da
NCBI Official Full Name
cytosolic phosphoenolpyruvate carboxykinase 1
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
PEPCK1; PEPCKC; PEPCK-C
  [Similar Products]
NCBI Protein Information
phosphoenolpyruvate carboxykinase, cytosolic [GTP]
UniProt Protein Name
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
PEPCK-C  [Similar Products]
UniProt Entry Name
PCKGC_HUMAN
NCBI Summary for PCK1
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for PCK1
PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; Carbohydrate Metabolism - pyruvate; Kinase, other; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol

Molecular Function: carboxylic acid binding; GDP binding; GTP binding; magnesium ion binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity

Biological Process: cellular response to potassium ion starvation; gluconeogenesis; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process from pyruvate; internal protein amino acid acetylation; oxaloacetate metabolic process; response to activity; response to insulin stimulus

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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