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anti-PLIN3 antibody :: Goat perilipin 3/TIP47 Polyclonal Antibody

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Catalog # MBS422908
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

perilipin 3/TIP47 (PLIN3), Polyclonal Antibody

Popular Item
Also Known As

Goat anti-perilipin 3/TIP47 (aa154-167) Antibody

Product Synonym Names
PLIN3; perilipin 3; M6PRBP1; MGC11117; MGC2012; PP17; TIP47; 47 kDa MPR-binding protein; cargo selection protein TIP47; mannose-6-phosphate receptor-binding protein 1; perilipin-3; placental protein 17; tail-interacting protein, 47 kD; PLIN3 antibody; perilipin 3 antibody; M6PRBP1 antibody; MGC11117 antibody; MGC2012 antibody; PP17 antibody; TIP47 antibody; 47 kDa MPR-binding protein antibody; cargo selection protein TIP47 antibody; mannose-6-phosphate receptor-binding protein 1 antibody; perilipin-3 antibody; placental protein 17 antibody; tail-interacting protein; 47 kD antibody; perilipin 3; TIP47; perilipin 3 / TIP47 (aa154-167)
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
434
Immunogen Sequence
TRGAVQSGVD KTKS
OMIM
602702
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen
Peptide with sequence C-TRGAVQSGVDKTKS, from the internal region of the protein sequence according to NP_005808.3; NP_001157661.1; NP_001157666.1.
Epitope
Internal region
Note
This antibody is expected to recognize all reported isoforms (NP_005808.3; NP_001157661.1; NP_001157666.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-PLIN3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PLIN3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-PLIN3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 48kDa+45kDa double bands observed in lysates of cell line HeLa and of Human Colon cancer (calculated MW of 47.1kDa according to NP_NP_005808.3, 46.9kDa according to NP_001157661.1 and 45.8kDa according to NP_001157666.1). The observed bands represent the three different isoforms. Please note the difference in band pattern intensities between HeLa and Colon cancer lysates. Recommended concentration: 0. 03-0.1ug/ml.

Western Blot (WB) of anti-PLIN3 antibody
(0. 03ug/ml) staining of HeLa (lane 1) and Human Colon cancer (lane two) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PLIN3 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PLIN3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001157661.1; NP_001157666.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,803 Da
NCBI Official Full Name
perilipin-3 isoform 1
NCBI Official Synonym Full Names
perilipin 3
NCBI Official Symbol
PLIN3  [Similar Products]
NCBI Official Synonym Symbols
PP17; TIP47; M6PRBP1
  [Similar Products]
NCBI Protein Information
perilipin-3
UniProt Protein Name
Perilipin-3
UniProt Synonym Protein Names
47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17
Protein Family
UniProt Gene Name
PLIN3  [Similar Products]
UniProt Synonym Gene Names
M6PRBP1; TIP47; 47 kDa MPR-binding protein; PP17  [Similar Products]
UniProt Entry Name
PLIN3_HUMAN
NCBI Summary for PLIN3
Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]
UniProt Comments for PLIN3
perilipin 3: a membrane-associated protein and a coat protein for lipid droplets (LDs). It has been described as a mannose-6-phosphate receptor (MPR) binding protein required for endosome-to-Golgi transport of MPRs, but this function has been disputed. Interacts with M6PR and IGF2R via their cytoplasmic domains. Affects hepatic lipid and glucose metabolism. Belongs to the perilipin family. 3 isoforms of the human protein are produced by alternative splicing. Isoform 2 may exist as a homodimer known as PP17C.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; cytosol; endosome; endosome membrane; Golgi apparatus; intracellular membrane-bound organelle; lipid particle; membrane; transport vesicle

Molecular Function: protein binding

Biological Process: vesicle-mediated transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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