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anti-PIH1D1 antibody :: Rabbit anti-Human PIH1D1 Polyclonal Antibody

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Catalog # MBS7000171
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

PIH1D1, Polyclonal Antibody

Popular Item
Full Product Name

PIH1D1 Antibody

Product Synonym Names
PIH1 domain-containing protein 1; PIH1D1; NOP17; Nucleolar protein 17 homolog
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
611480
3D Structure
ModBase 3D Structure for Q9NWS0
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Affinity Purified
Form/Format
Liquid
Immunogen
Recombinant human PIH1 domain-containing protein 1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-168777 / sc-168778
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PIH1D1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PIH1D1 antibody
Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles (PubMed:17636026). Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription (PubMed:22368283, PubMed:24036451). Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1 (PubMed:20864032). Positively regulates the assembly and activity of the mTORC1 complex (PubMed:24036451).
Applications Tested/Suitable for anti-PIH1D1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PIH1D1 antibody
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200

Immunohistochemistry (IHC) of anti-PIH1D1 antibody
Immunohistochemistry of paraffin-embedded human placenta tissue using MBS7000171 at dilution of 1:100
anti-PIH1D1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-PIH1D1 antibody
Immunofluorescence staining of Hela cells cells with MBS7000171 at 1:200,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
anti-PIH1D1 antibody Immunofluorescence (IF) image
Immunoprecipitation (IP) of anti-PIH1D1 antibody
Immunoprecipitating PIH1D1 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1ug)instead of MBS7000171 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS7000171 (6ug)+ Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (10ug)

anti-PIH1D1 antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for PIH1D1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,376 Da
NCBI Official Full Name
PIH1 domain-containing protein 1
NCBI Official Synonym Full Names
PIH1 domain containing 1
NCBI Official Symbol
PIH1D1  [Similar Products]
NCBI Official Synonym Symbols
NOP17
  [Similar Products]
NCBI Protein Information
PIH1 domain-containing protein 1
UniProt Protein Name
PIH1 domain-containing protein 1
UniProt Synonym Protein Names
Nucleolar protein 17 homolog
UniProt Gene Name
PIH1D1  [Similar Products]
UniProt Synonym Gene Names
NOP17  [Similar Products]
UniProt Entry Name
PIHD1_HUMAN
UniProt Comments for PIH1D1
PIH1D1: a protein of unknown function.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytoplasm; nucleolus; nucleus

Molecular Function: ATPase binding; histone binding; phosphoprotein binding; protein binding; protein kinase binding

Biological Process: box C/D snoRNP assembly; chromatin remodeling; epithelial cell differentiation; positive regulation of protein complex assembly; regulation of histone H3-K4 methylation; snoRNA localization; transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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