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anti-PPARD antibody :: Rabbit anti-Human PPARd Polyclonal Antibody

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Catalog # MBS612577
Unit / Price
  0.1 mg  /  $740 +1 FREE 8GB USB
anti-PPARD antibody
Product Name

PPARd, Polyclonal Antibody

Also Known As

PPARd (Peroxisome Proliferator Activated Receptor, delta)

Product Synonym Names
Anti -PPARd (Peroxisome Proliferator Activated Receptor, delta)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 6; NC_000006.11 (35310335..35395968). Location: 6p21.2
OMIM
600409
3D Structure
ModBase 3D Structure for Q03181
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
Recognizes the human PPAR delta. Expected to react with mouse and rat based on 100% sequence homology. Crossreactivity with PPAR delta from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. No reactivity is expected with other subtypesof PPAR.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Immunogen
A synthetic peptide, MEQPQEETPEAREE, corresponding to an internal region of mouse PPAR delta.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-PPARD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PPARD antibody
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and antidiabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPARgamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
Applications Tested/Suitable for anti-PPARD antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-PPARD antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:2000-1:8000
Western Blot: 1:500-1:2000.
NCBI/Uniprot data below describe general gene information for PPARD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,903 Da[Similar Products]
NCBI Official Full Name
peroxisome proliferator-activated receptor delta isoform 4
NCBI Official Synonym Full Names
peroxisome proliferator-activated receptor delta
NCBI Official Symbol
PPARD  [Similar Products]
NCBI Official Synonym Symbols
FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB; MGC3931
  [Similar Products]
NCBI Protein Information
peroxisome proliferator-activated receptor delta; PPAR-beta; PPAR-delta; OTTHUMP00000016256; OTTHUMP00000016257; nuclear hormone receptor 1; nuclear receptor subfamily 1 group C member 2; peroxisome proliferator-activated receptor beta; peroxisome proliferator-activated nuclear receptor beta/delta variant 2
UniProt Protein Name
Peroxisome proliferator-activated receptor delta
UniProt Synonym Protein Names
NUCI; Nuclear hormone receptor 1; NUC1; Nuclear receptor subfamily 1 group C member 2; Peroxisome proliferator-activated receptor beta
UniProt Gene Name
PPARD  [Similar Products]
UniProt Synonym Gene Names
NR1C2; PPARB  [Similar Products]
UniProt Entry Name
PPARD_HUMAN
NCBI Summary for PPARD
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
UniProt Comments for PPARD
PPAR-delta: Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand. Belongs to the nuclear hormone receptor family. NR1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: NF-kappaB binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; steroid hormone receptor activity; drug binding; transcription factor activity; lipid binding

Biological Process: proteoglycan metabolic process; wound healing; negative regulation of smooth muscle cell proliferation; heart development; negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; positive regulation of epidermis development; negative regulation of smooth muscle cell migration; glucose transport; negative regulation of transcription from RNA polymerase II promoter; decidualization; positive regulation of vasodilation; vitamin A metabolic process; response to vitamin A; positive regulation of cell proliferation; response to glucose stimulus; axon ensheathment; cell differentiation; negative regulation of epithelial cell proliferation; transcription initiation from RNA polymerase II promoter; cholesterol metabolic process; generation of precursor metabolites and energy; intracellular receptor-mediated signaling pathway; glucose metabolic process; positive regulation of insulin secretion; fatty acid catabolic process; keratinocyte proliferation; keratinocyte migration; cell-substrate adhesion; phospholipid biosynthetic process; regulation of transcription from RNA polymerase II promoter; positive regulation of phosphoinositide 3-kinase cascade; cell proliferation; fatty acid beta-oxidation; negative regulation of inflammatory response; mRNA transcription; positive regulation of fat cell differentiation; steroid hormone mediated signaling; gene expression; response to activity; negative regulation of cell growth; lipid metabolic process; negative regulation of transcription, DNA-dependent; embryo implantation; fatty acid transport; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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