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anti-PRKAA2 antibody :: Rabbit anti-Human PRKAA2 Polyclonal Antibody

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Catalog # MBS7045064
Unit / Price
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  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

PRKAA2, Polyclonal Antibody

Popular Item
Full Product Name

PRKAA2 Antibody

Product Synonym Names
5'-AMP-activated protein kinase catalytic subunit alpha-2; Acetyl-CoA carboxylase kinase (EC:2.7.11.27)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
600497
3D Structure
ModBase 3D Structure for P54646
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Species
Human
Immunogen
Recombinant human 5'-AMP-activated protein kinase catalytic subunit alpha-2 protein
Conjugate
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-19131 / sc-19129
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PRKAA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PRKAA2 antibody
Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. Involved in insulin receptor/INSR internalization (PubMed:25687571). AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. Plays an important role in the differential regulation of pro-autophagy (composed of PIK3C3, BECN1, PIK3R4 and UVRAG or ATG14) and non-autophagy (composed of PIK3C3, BECN1 and PIK3R4) complexes, in response to glucose starvation. Can inhibit the non-autophagy complex by phosphorylating PIK3C3 and can activate the pro-autophagy complex by phosphorylating BECN1 (By similarity).
Applications Tested/Suitable for anti-PRKAA2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunohistochemistry (IHC) of anti-PRKAA2 antibody
Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045064 at dilution of 1:100
anti-PRKAA2 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-PRKAA2 antibody
Immunofluorescent analysis of MCF7 cells using MBS7045064 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-PRKAA2 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-PRKAA2 antibody
Western Blot
Positive WB detected in:Hela whole cell lysate,Mouse heart tissue,Mouse lung tissue,Mouse kidney tissue,Mouse brain tissue,Mouse skeletal muscle tissue
All lanes: PRKAA2 antibody at 2.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa
Observed band size: 63,48 kDa

anti-PRKAA2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PRKAA2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
62,320 Da
NCBI Official Full Name
5'-AMP-activated protein kinase catalytic subunit alpha-2
NCBI Official Synonym Full Names
protein kinase AMP-activated catalytic subunit alpha 2
NCBI Official Symbol
PRKAA2  [Similar Products]
NCBI Official Synonym Symbols
AMPK; AMPK2; PRKAA; AMPKa2
  [Similar Products]
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-2
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-2
UniProt Synonym Protein Names
Acetyl-CoA carboxylase kinase (EC:2.7.11.27
UniProt Gene Name
PRKAA2  [Similar Products]
UniProt Synonym Gene Names
AMPK; AMPK2; HMGCR kinase  [Similar Products]
UniProt Entry Name
AAPK2_HUMAN
NCBI Summary for PRKAA2
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq, Jul 2008]
UniProt Comments for PRKAA2
AMPKA2: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of metabolic enzymes and transcription regulators. Regulates fatty acid synthesis by phosphorylating acetyl-CoA carboxylase. Regulates cholesterol synthesis by phosphorylating and inactivating hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Salicylate/aspirin directly activates kinase activity. Studies in the mouse suggest that AMPK2 may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Protein type: EC 2.7.11.1; Autophagy; Protein kinase, CAMK; Kinase, protein; EC 2.7.11.31; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.27; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: AMP-activated protein kinase complex; cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: AMP-activated protein kinase activity; chromatin binding; histone serine kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: carnitine shuttle; cell cycle arrest; cellular response to glucose starvation; cellular response to nutrient levels; fatty acid homeostasis; glucose homeostasis; lipid biosynthetic process; macroautophagy; negative regulation of apoptosis; negative regulation of TOR signaling pathway; positive regulation of autophagy; positive regulation of glycolysis; positive regulation of macroautophagy; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of fatty acid biosynthetic process; regulation of macroautophagy; response to stress; signal transduction
Precautions
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Disclaimer
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