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anti-HPGD antibody :: Goat Prostaglandin dehydrogenase 1 Polyclonal Antibody

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Catalog # MBS421451
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

Prostaglandin dehydrogenase 1 (HPGD), Polyclonal Antibody

Popular Item
Also Known As

Goat anti-Prostaglandin dehydrogenase 1 Antibody

Product Synonym Names
HPGD; Prostaglandin dehydrogenase 1; PGDH1; 15-PGDH; hydroxyprostaglandin dehydrogenase 15-(NAD); PGDH; SDR36C1; NAD+-dependent 15-hydroxyprostaglandin dehydrogenase; short chain dehydrogenase/reductase family 36C, member 1; Prostaglandin dehydrogenase 1 antibody; HPGD antibody; PGDH1 antibody; 15-PGDH antibody; hydroxyprostaglandin dehydrogenase 15-(NAD) antibody; Prostaglandin dehydrogenase 1; Prostaglandin dehydrogenase 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
266
Immunogen Sequence
DYDTTPFQAK TQ
OMIM
119900
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DYDTTPFQAKTQ, from the C Terminus of the protein sequence according to NP_000851.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-HPGD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HPGD antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-HPGD antibody
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Immunohistochemistry: In paraffin embedded Human Colon shows strong cytoplasm staining of enterocytes. Recommended concentration, 2-4ug/ml.
Western Blot: Experiments gave bands at approx 26kDa and 50kDa in Human Duodenum lysates after 0.1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (PMID: 11889207). This protein has a calculated MW of 29. 0kDa according to NP_000851. Recommended concentration: 0.1-1ug/ml.

Western Blot (WB) of anti-HPGD antibody
(0.1ug/ml) staining of Human Duodenum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-HPGD antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HPGD antibody
(2.5ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-HPGD antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HPGD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
21,526 Da
NCBI Official Full Name
15-hydroxyprostaglandin dehydrogenase
NCBI Official Synonym Full Names
hydroxyprostaglandin dehydrogenase 15-(NAD)
NCBI Official Symbol
NCBI Official Synonym Symbols
PGDH; PGDH1; PHOAR1; 15-PGDH; SDR36C1
  [Similar Products]
NCBI Protein Information
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
UniProt Protein Name
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
UniProt Synonym Protein Names
Prostaglandin dehydrogenase 1; Short chain dehydrogenase/reductase family 36C member 1
UniProt Gene Name
UniProt Synonym Gene Names
PGDH1; SDR36C1; 15-PGDH  [Similar Products]
UniProt Entry Name
PGDH_HUMAN
NCBI Summary for HPGD
This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Comments for HPGD
HPGD: Prostaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells. Defects in HPGD are the cause of hypertrophic osteoarthropathy, primary, autosomal recessive, type 1 (PHOAR1). A disease characterized by digital clubbing, periostosis, acroosteolysis, painful joint enlargement, and variable features of pachydermia that include thickened facial skin and a thickened scalp. Other developmental anomalies include delayed closure of the cranial sutures and congenital heart disease. Defects in HPGD are the cause of cranioosteoarthropathy (COA). A form of osterarthropathy characterized by swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature. Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC); also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oxidoreductase; Tumor suppressor; EC 1.1.1.141

Chromosomal Location of Human Ortholog: 4q34-q35

Cellular Component: basolateral plasma membrane; cytoplasm; cytosol; nucleoplasm

Molecular Function: 15-hydroxyprostaglandin dehydrogenase (NAD+) activity; catalytic activity; NAD binding; prostaglandin E receptor activity; protein homodimerization activity

Biological Process: female pregnancy; lipoxygenase pathway; negative regulation of cell cycle; ovulation; parturition; prostaglandin metabolic process; transforming growth factor beta receptor signaling pathway

Disease: Digital Clubbing, Isolated Congenital; Hypertrophic Osteoarthropathy, Primary, Autosomal Recessive, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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