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anti-PAR4 antibody :: Rabbit anti-Human Protease Activated Receptor 4 Polyclonal Antibody

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Catalog # MBS2028760 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $165 +1 FREE 8GB USB
  0.1 mg  /  $210 +1 FREE 8GB USB
  0.2 mg  /  $310 +1 FREE 8GB USB
  1 mg  /  $725 +1 FREE 8GB USB
Product Name

Protease Activated Receptor 4 (PAR4), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Protease Activated Receptor 4 (PAR4)

Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Unconjugated Antibody: Protease Activated Receptor 4 (MBS2028760)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Protease Activated Receptor 4 (MBS2028760)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF080214 mRNA
3D Structure
ModBase 3D Structure for Q96RI0
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a Rabbit polyclonal antibody raised against PAR4. It has been selected for its ability to recognize PAR4 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific Affinity Chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
Concentration
200µg/mL (lot specific)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant PAR4 (Gly48~Gln385) expressed in E.coli
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038319
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Other Notes
Small volumes of anti-PAR4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PAR4 antibody
WB; IHC; ICC; IP
Application Notes for anti-PAR4 antibody
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL

Optimal working dilutions must be determined by end user

Western Blot (WB) of anti-PAR4 antibody
(Figure. Western Blot; Sample: Recombinant PAR4, Human.)
anti-PAR4 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PAR4 antibody
(Figure. DAB staining on IHC-P; Samples: Human Lung Cancer Tissue.)
anti-PAR4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PAR4 antibody
(Figure. DAB staining on IHC-P; Samples: Human Kidney Tissue.)
anti-PAR4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PAR4 antibody
(Figure. DAB staining on IHC-P; Samples: Human Stomach Tissue.)
anti-PAR4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PAR4 antibody
(Figure. DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)
anti-PAR4 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PAR4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,133 Da
NCBI Official Full Name
proteinase-activated receptor 4 preproprotein
NCBI Official Synonym Full Names
F2R like thrombin or trypsin receptor 3
NCBI Official Symbol
F2RL3  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
proteinase-activated receptor 4
UniProt Protein Name
Proteinase-activated receptor 4
UniProt Synonym Protein Names
Coagulation factor II receptor-like 3; Thrombin receptor-like 3
UniProt Gene Name
F2RL3  [Similar Products]
UniProt Synonym Gene Names
PAR4; PAR-4  [Similar Products]
NCBI Summary for PAR4
This gene encodes a member of the protease-activated receptor subfamily, part of the G-protein coupled receptor 1 family of proteins. The encoded receptor is proteolytically processed to reveal an extracellular N-terminal tethered ligand that binds to and activates the receptor. This receptor plays a role in blood coagulation, inflammation and response to pain. Hypomethylation at this gene may be associated with lung cancer in human patients. [provided by RefSeq, Sep 2016]
UniProt Comments for PAR4
PAR4: a G-protein coupled receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Plays a role in platelets activation. Thrombin requires PAR4 for full spreading on a fibrinogen matrix. Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets. A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: extracellular region; integral component of plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; thrombin receptor activity

Biological Process: blood coagulation; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); phospholipase C-activating G-protein coupled receptor signaling pathway; platelet activation; platelet dense granule organization and biogenesis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of Rho protein signal transduction; response to wounding; signal transduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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