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anti-PSMa5 antibody :: Rabbit anti-Human Proteasome Subunit Alpha Type 5 Polyclonal Antibody

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Catalog # MBS2027800 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Proteasome Subunit Alpha Type 5 (PSMa5), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Proteasome Subunit Alpha Type 5 (PSMa5)

Matching Pairs
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Matching Pairs
Unconjugated Antibody: Proteasome Subunit Alpha Type 5 (MBS2027800)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Proteasome Subunit Alpha Type 5 (MBS2027800)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
X61970 mRNA
3D Structure
ModBase 3D Structure for P28066
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against PSMa5. It has beenselected for its ability to recognize PSMa5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
PSMa5 (Met1~Ile241)
Organism Species
Homo sapiens (Human)
Conjugate
No Conjugate
Immunogen
Recombinant PSMa5 (Met1~Ile241) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2077666
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-PSMa5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-PSMa5 antibody
Applications Tested/Suitable for anti-PSMa5 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-PSMa5 antibody
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) Knockout Verification of anti-PSMa5 antibody
Knockout Verification: Lane 1: Wild-type Hela cell lysate; Lane 2: PSMa5 knockout Hela cell lysate; Predicted MW: 26,20kd Observed MW: 27kd Primary Ab: 1ug/ml Rabbit Anti-Human PSMa5 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-PSMa5 antibody Western Blot (WB) Knockout Verification (WB) image
Western Blot (WB) of anti-PSMa5 antibody
Western Blot; Sample: Lane1: Human Lung Tissue; Lane2: Human Hela Cells.
anti-PSMa5 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-PSMa5 antibody
Western Blot; Sample: Recombinant PSMa5, Human.
anti-PSMa5 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PSMa5 antibody
DAB staining on IHC-P; Samples: Human Stomach Tissue.
anti-PSMa5 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PSMa5 antibody
DAB staining on IHC-P; Samples: Human Brain Tissue
anti-PSMa5 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PSMa5 antibody
DAB staining on IHC-P; Samples: Human Kidney Tissue
anti-PSMa5 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PSMa5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,035 Da
NCBI Official Full Name
proteasome subunit alpha type-5 isoform 2
NCBI Official Synonym Full Names
proteasome subunit alpha 5
NCBI Official Symbol
PSMA5  [Similar Products]
NCBI Official Synonym Symbols
PSC5; ZETA
  [Similar Products]
NCBI Protein Information
proteasome subunit alpha type-5
UniProt Protein Name
Proteasome subunit alpha type-5
UniProt Synonym Protein Names
Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain
UniProt Gene Name
PSMA5  [Similar Products]
NCBI Summary for PSMa5
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]
UniProt Comments for PSMa5
PSMA5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain. May be the target of the transcriptional activator NFE2L2. Expressed in fetal brain. Belongs to the peptidase T1A family.

Protein type: EC 3.4.25.1; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytosol; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; nucleoplasm; nucleus; proteasome complex; proteasome core complex; proteasome core complex, alpha-subunit complex; secretory granule lumen

Molecular Function: protein binding; threonine endopeptidase activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; Fc-epsilon receptor signaling pathway; MAPK cascade; negative regulation of canonical Wnt signaling pathway; negative regulation of G2/M transition of mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; neutrophil degranulation; NIK/NF-kappaB signaling; positive regulation of canonical Wnt signaling pathway; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; post-translational protein modification; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein polyubiquitination; regulation of cellular amino acid metabolic process; regulation of hematopoietic stem cell differentiation; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with anti-PSMa5 antibody
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