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anti-PUF60 antibody :: Rabbit anti-Human PUF60 Polyclonal Antibody

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Catalog # MBS9208219
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

PUF60, Polyclonal Antibody

Popular Item
Full Product Name

PUF60 Antibody (N-term)

Product Synonym Names
Poly(U)-binding-splicing factor PUF60; 60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1; PUF60; FIR; ROBPI; SIAHBP1
Antibody/Peptide Pairs
PUF60 peptide (MBS9220723) is used for blocking the activity of PUF60 antibody (MBS9208219)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
15-43
OMIM
604819
3D Structure
ModBase 3D Structure for Q9UHX1
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Specificity
This PUF60 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human PUF60.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (lot specific)
Antigen Type
Synthetic Peptide
ID
RB34229
Antigen Source
Human
Function
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
Cellular Location
Nucleus. Note=Colocalizes partially with TROVE2
Tissue Location
Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-PUF60 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PUF60 antibody
The protein encoded by this gene is a Ro RNP-binding
protein. It interacts with Ro RNPs and their interaction is thought
to represent a gain of function for Ro RNPs. This protein also
forms a ternary complex with far upstream element (FUSE) and
FUSE-binding protein. It can repress a c-myc reporter via the FUSE.
It is also known to target transcription factor IIH and inhibit
activated transcription. This gene is implicated in the xeroderma
pigmentosum disorder. There are two alternatively spliced
transcript variants of this gene encoding different isoforms. There
seems to be evidence of multiple polyadenylation sites for this
gene.
Applications Tested/Suitable for anti-PUF60 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-PUF60 antibody
WB~~1:1000

Western Blot (WB) of anti-PUF60 antibody
PUF60 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUF60 antibody detected the PUF60 protein (arrow).
anti-PUF60 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for PUF60. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59875
NCBI Official Full Name
poly(U)-binding-splicing factor PUF60 isoform c
NCBI Official Synonym Full Names
poly-U binding splicing factor 60KDa
NCBI Official Symbol
PUF60  [Similar Products]
NCBI Official Synonym Symbols
FIR; VRJS; RoBPI; SIAHBP1
  [Similar Products]
NCBI Protein Information
poly(U)-binding-splicing factor PUF60
UniProt Protein Name
Poly(U)-binding-splicing factor PUF60
UniProt Synonym Protein Names
60 kDa poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; FBP-interacting repressor; Ro-binding protein 1; RoBP1; Siah-binding protein 1; Siah-BP1
UniProt Gene Name
PUF60  [Similar Products]
UniProt Synonym Gene Names
FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1  [Similar Products]
UniProt Entry Name
PUF60_HUMAN
NCBI Summary for PUF60
This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
UniProt Comments for PUF60
FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor; RNA-binding; RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: identical protein binding; protein binding; DNA binding; nucleotide binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing

Disease: Verheij Syndrome
Product References and Citations for anti-PUF60 antibody
Hsiao, H.H., et al. Biochemistry 49(22):4620-4634(2010)
Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)
Gao, J., et al. Genomics 91(4):347-355(2008)
Hastings, M.L., et al. PLoS ONE 2 (6), E538 (2007) :
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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