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anti-PKM2 antibody :: Rabbit anti-Human Pyruvate Kinase Polyclonal Antibody

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Catalog # MBS2026646 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $165 +1 FREE 8GB USB
  0.1 mg  /  $210 +1 FREE 8GB USB
  0.2 mg  /  $310 +1 FREE 8GB USB
  1 mg  /  $725 +1 FREE 8GB USB
Product Name

Pyruvate Kinase (PKM2), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)

Matching Pairs
Matching Pairs
Unconjugated Antibody: Pyruvate Kinase (MBS2026646)
APC-CY7 Conjugated Antibody: Pyruvate Kinase (PKM2) (MBS2044621)
Matching Pairs
Unconjugated Antibody: Pyruvate Kinase (MBS2026646)
PE Conjugated Antibody: Pyruvate Kinase (PKM2) (MBS2044623)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
M23725 mRNA
3D Structure
ModBase 3D Structure for P14618
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a Rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific Affinity Chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
Concentration
200µg/mL (lot specific)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant PKM2 (Arg294~Phe470) expressed in E.coli
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2044625
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-PKM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-PKM2 antibody
WB; IHC; ICC; IP
Application Notes for anti-PKM2 antibody
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL

Optimal working dilutions must be determined by end user

Western Blot (WB) of anti-PKM2 antibody
Western Blot: Sample: Lane1: Human A549 Cells; Lane2: Human HepG2 Cells; Lane3: Human Hela Cells.
anti-PKM2 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-PKM2 antibody
Western Blot: Sample: Recombinant PKM2, Human.
anti-PKM2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PKM2 antibody
DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue.
anti-PKM2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PKM2 antibody
DAB staining on IHC-P; Samples: Human Prostate Gland Tissue
anti-PKM2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PKM2 antibody
DAB staining on IHC-P; Samples: Human Breast Cancer Tissue
anti-PKM2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,273 Da
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase M1/2
NCBI Official Symbol
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
  [Similar Products]
NCBI Protein Information
pyruvate kinase PKM
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1  [Similar Products]
NCBI Summary for PKM2
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
UniProt Comments for PKM2
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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