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anti-RPTOR antibody :: Rabbit anti-Human Raptor / Mip1 Polyclonal Antibody

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Catalog # MBS241455
Unit / Price
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  0.05 mL  /  $530 +1 FREE 8GB USB
Product Name

Raptor / Mip1 (RPTOR), Polyclonal Antibody

Full Product Name

Rabbit Polyclonal (IgG) to Human Raptor / Mip1

Product Synonym Names
Anti-Raptor / Mip1 Antibody IHC-plus; KOG1; KIAA1303; RAPTOR; Mip1; Raptor; Human Raptor; Mip1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
607130
3D Structure
ModBase 3D Structure for Q8N122
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
Peptide-KLH
Purity/Purification
Immunoaffinity Purified
Form/Format
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Target Species
Human
Immunogen Description
Synthetic peptide from human RPTOR / Raptor.
Immunogen Type
Synthetic peptide
Immunogen
Raptor / Mip1 antibody was raised against synthetic peptide from human RPTOR / Raptor.
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-RPTOR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-RPTOR antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-RPTOR antibody
IHC-P (1:50), WB
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-RPTOR antibody
Anti-RPTOR / Raptor antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
anti-RPTOR antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-RPTOR antibody
293T cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Raptor antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
anti-RPTOR antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for RPTOR. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
131,515 Da
NCBI Official Full Name
regulatory-associated protein of mTOR isoform 1
NCBI Official Synonym Full Names
regulatory associated protein of MTOR, complex 1
NCBI Official Symbol
RPTOR  [Similar Products]
NCBI Official Synonym Symbols
KOG1; Mip1
  [Similar Products]
NCBI Protein Information
regulatory-associated protein of mTOR; raptor; p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
UniProt Protein Name
Regulatory-associated protein of mTOR
UniProt Synonym Protein Names
p150 target of rapamycin (TOR)-scaffold protein
UniProt Gene Name
RPTOR  [Similar Products]
UniProt Synonym Gene Names
KIAA1303; RAPTOR; Raptor  [Similar Products]
UniProt Entry Name
RPTOR_HUMAN
NCBI Summary for RPTOR
This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
UniProt Comments for RPTOR
Raptor: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Interacts with MTOR. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity. Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Belongs to the WD repeat RAPTOR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cell soma; lysosomal membrane; lysosome; cytoplasm; dendrite; cytosol

Molecular Function: protein binding; protein complex binding; protein kinase binding

Biological Process: cellular response to nutrient levels; TOR signaling pathway; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase III promoter; positive regulation of endothelial cell proliferation; cell growth; cell cycle arrest; regulation of cell size; positive regulation of TOR signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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