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anti-RELA antibody :: Rabbit anti-Human RELA / NFKB p65 Polyclonal Antibody

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Catalog # MBS240395
Unit / Price
  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

RELA / NFKB p65, Polyclonal Antibody

Also Known As

Rabbit Polyclonal to Human RELA / NFKB p65

Product Synonym Names
Anti-RELA / NFKB p65 Antibody IHC-plus; RELA; NF-kappa-B p65delta3; NFKB3; p65; Transcription factor p65; Human RELA; NFKB p65
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
3D Structure
ModBase 3D Structure for Q04206
Species Reactivity
Residues surrounding S536 of human p65 (RelA) protein.
Immunoaffinity Purified
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
1 mg/ml (lot specific)
Target Species
Immunogen Description
Synthetic peptide from human RELA.
Immunogen Type
Synthetic peptide
RELA / NFKB p65 antibody was raised against synthetic peptide from human RELA.
Preparation and Storage
+4 degree C or -20 degree C, Avoid repeated freezing and thawing.
Other Notes
Small volumes of anti-RELA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RELA antibody
NFKB1 or NFKB2 bind to REL, RELA, or RELB to form the NFKB complex. The NFKB1/RELA heterodimer is the most abundant form of NFKB. The NFKB complex is inactivated by I-kappa-B proteins that keep it trapped in the cytoplasm. Phosphorylation of serine residues on the I-kappa B proteins mark them for ubiquitination and destruction, and NFKB translocates to the nucleus where it binds DNA.
Applications Tested/Suitable for anti-RELA antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes for anti-RELA antibody
ELISA (1:1000 - 1:6000), IHC-P (5 ug/ml), WB (1:200 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...

Immunohistochemistry (IHC) of anti-RELA antibody
Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-RELA antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-RELA antibody
Anti-p65 (RelA) pS536 Antibody - Western Blot. Western blot of affinity purified anti-p65 (RelA) pS536 antibody shows detection of p65 phosphorylated at S536. The control blot (left lane) contains 100 ng of purified p65-GST fusion protein. The band is seen (right lane) when this protein is phosphorylated at S536 by IKKbeta. Personal Communication. J. Brady, NCI, Bethesda, MD.
anti-RELA antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for RELA. It may not necessarily be applicable to this product.
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
59,910 Da
NCBI Official Full Name
transcription factor p65 isoform 1
NCBI Official Synonym Full Names
v-rel avian reticuloendotheliosis viral oncogene homolog A
NCBI Official Symbol
NCBI Official Synonym Symbols
p65; NFKB3
  [Similar Products]
NCBI Protein Information
transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
UniProt Protein Name
Transcription factor p65
UniProt Synonym Protein Names
Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NFKB3  [Similar Products]
UniProt Entry Name
NCBI Summary for RELA
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Comments for RELA
NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; identical protein binding; protein binding; DNA binding; transcription activator binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; transcription factor activity; transcription factor binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; response to cAMP; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; response to morphine; negative regulation of insulin receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; response to insulin stimulus; toll-like receptor 5 signaling pathway; response to organic substance; hair follicle development; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; aging; response to drug; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; membrane protein intracellular domain proteolysis; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; acetaldehyde metabolic process; liver development; response to amino acid stimulus; toll-like receptor 2 signaling pathway; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; positive regulation of interleukin-12 biosynthetic process; response to mechanical stimulus; response to muramyl dipeptide; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; response to cobalamin; negative regulation of apoptosis
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