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anti-RPS6KA1 antibody :: Rabbit anti-Human RPS6K1 Polyclonal Antibody

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Catalog # MBS150766
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

RPS6K1 (RPS6KA1), Polyclonal Antibody

Also Known As

RPS6K1 Antibody

Product Synonym Names
RPS6K1; RSK; HU-1; RSK1; MAPKAPK1A; Ribosomal protein S6 kinase alpha-1; 90 kDa ribosomal protein S6 kinase 1; S6K-alpha-1; ribosomal protein S6 kinase, 90kDa, polypeptide 1
Antibody/Peptide Pairs
RPS6K1 peptide (MBS152754) is used for blocking the activity of RPS6K1 antibody (MBS150766)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
735
OMIM
601684
3D Structure
ModBase 3D Structure for Q15418
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
RPS6K1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
RPS6K1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human RPS6K1.
Buffer
RPS6K1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
RPS6K1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-RPS6KA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-RPS6KA1 antibody
RPS6K1 Antibody: Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GbetaL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth. RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.
Applications Tested/Suitable for anti-RPS6KA1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-RPS6KA1 antibody
RPS6K1antibody can be used for the detection of RPS6K1 by Western blot at 2.5 - 10 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-RPS6KA1 antibody
Western blot analysis of RPS6K1 in Jurkat cell lysate with RPS6K1 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.
anti-RPS6KA1 antibody Western Blot (WB) (WB) image
Immunocytochemistry (ICC) of anti-RPS6KA1 antibody
Immunocytochemistry of RPS6K1 in Jurkat cells with RPS6K1 antibody at 10 μg/mL.
anti-RPS6KA1 antibody Immunocytochemistry (ICC) (ICC) image
Immunofluorescence (IF) of anti-RPS6KA1 antibody
Immunofluorescence of RP S6 Kinase in Jurkat cells with RP S6 Kinase antibody at 20 μg/mL.
anti-RPS6KA1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for RPS6KA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
72,698 Da
NCBI Official Full Name
ribosomal protein S6 kinase 1
NCBI Official Synonym Full Names
ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol
RPS6KA1  [Similar Products]
NCBI Official Synonym Symbols
RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
  [Similar Products]
NCBI Protein Information
ribosomal protein S6 kinase alpha-1; RSK-1; p90S6K; p90RSK1; p90-RSK 1; MAPKAPK-1a; S6K-alpha 1; S6K-alpha-1; MAPKAP kinase 1a; ribosomal S6 kinase 1; MAPK-activated protein kinase 1a; ribosomal protein S6 kinase alpha 1; 90 kDa ribosomal protein S6 kinase 1; MAP kinase-activated protein kinase 1a; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1)
UniProt Protein Name
Ribosomal protein S6 kinase alpha-1
UniProt Synonym Protein Names
90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1
UniProt Gene Name
RPS6KA1  [Similar Products]
UniProt Synonym Gene Names
MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1  [Similar Products]
UniProt Entry Name
KS6A1_HUMAN
NCBI Summary for RPS6KA1
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Comments for RPS6KA1
p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Translation; EC 2.7.11.1; AGC group; RSK family; RSK subfamily

Chromosomal Location of Human Ortholog: 1p

Cellular Component: nucleoplasm; cytoplasm; spindle; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; caspase inhibitor activity; protein serine/threonine/tyrosine kinase activity; magnesium ion binding; ATP binding

Biological Process: regulation of transcription in response to stress; axon guidance; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; regulation of translation in response to stress; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; negative regulation of caspase activity; signal transduction; cell cycle; protein amino acid phosphorylation; positive regulation of cell growth; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; synaptic transmission; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; positive regulation of cell differentiation; toll-like receptor 4 signaling pathway; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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