• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-SDHB antibody :: Goat SDHB Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS421127
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

SDHB, Polyclonal Antibody

Full Product Name

Goat anti-SDHB Antibody

Product Synonym Names
Goat Anti-SDHB, Biotinylated Antibody; SDHB; IP; SDH; SDH1; SDHIP; succinate dehydrogenase complex, subunit B, iron sulfur (Ip); OTTHUMP00000044624; iron-sulfur subunit; FLJ92337; PGL4; SDH2; SDHIP; SDHB antibody; IP antibody; SDH antibody; SDH1 antibody; SDHIP antibody; succinate dehydrogenase complex; subunit B; iron sulfur (Ip) antibody; OTTHUMP00000044624 antibody; iron-sulfur subunit antibody; FLJ92337 antibody; SDH2 antibody; SDHIP antibody
Matching Pairs
Unconjugated Antibody: SDHB (MBS421127)
Biotin Conjugated Antibody: SDHB (MBS423534)
Antibody/Peptide Pairs
SDHB peptide (MBS425682) is used for blocking the activity of SDHB antibody (MBS421127)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
ATYKEKKASV
OMIM
115310
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-ATYKEKKASV, from the C Terminus of the protein sequence according to NP_002991.2.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-SDHB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SDHB antibody
Peptide ELISA (EIA), Western Blot (WB), ELISA (EIA)
Application Notes for anti-SDHB antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 28kDa band observed in Human Liver lysates (calculated MW of 31.6kDa according to NP_002991.1). Recommended concentration: 0. 03-0.1ug/ml.
ELISA: Customer successfully used this product in quantitative ELISA on HepG2 lysates.

Western Blot (WB) of anti-SDHB antibody
(0. 03ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SDHB antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SDHB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
31,630 Da
NCBI Official Full Name
succinate dehydrogenase
NCBI Official Synonym Full Names
succinate dehydrogenase complex iron sulfur subunit B
NCBI Official Symbol
NCBI Official Synonym Symbols
IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
  [Similar Products]
NCBI Protein Information
succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
UniProt Protein Name
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
UniProt Synonym Protein Names
Iron-sulfur subunit of complex II; Ip
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
SDH; SDH1; Ip  [Similar Products]
UniProt Entry Name
SDHB_HUMAN
NCBI Summary for SDHB
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]
UniProt Comments for SDHB
SDHB: Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC). A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent. Defects in SDHB are the cause of paragangliomas type 4 (PGL4). A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion. Paragangliomas are most commonly located in the head and neck region, specifically at the carotid bifurcation, the jugular foramen, the vagal nerve, and in the middle ear. Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS); also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. Defects in SDHB are a cause of Cowden-like syndrome (CWDLS). Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.

Protein type: EC 1.3.5.1; Carbohydrate Metabolism - citrate (TCA) cycle; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 1p36.1-p35

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex II; mitochondrion; nucleoplasm; plasma membrane

Molecular Function: 2 iron, 2 sulfur cluster binding; 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; electron carrier activity; metal ion binding; protein binding; succinate dehydrogenase (ubiquinone) activity; ubiquinone binding

Biological Process: aerobic respiration; succinate metabolic process; tricarboxylic acid cycle

Disease: Cowden Syndrome 2; Gastrointestinal Stromal Tumor; Paraganglioma And Gastric Stromal Sarcoma; Paragangliomas 4; Pheochromocytoma
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000