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anti-SHMT2 antibody :: Rabbit anti-Human Serine Hydroxymethyltransferase 2 Polyclonal Antibody

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Catalog # MBS2027992 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Serine Hydroxymethyltransferase 2 (SHMT2), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)

Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AK315916 mRNA
3D Structure
ModBase 3D Structure for P34897
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against SHMT2. It has beenselected for its ability to recognize SHMT2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
SHMT2 (Asn30~His504)
Organism Species
Homo sapiens (Human)
Conjugate
No Conjugate
Immunogen
Recombinant SHMT2 (Asn30~His504) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074179
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-SHMT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-SHMT2 antibody
Applications Tested/Suitable for anti-SHMT2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-SHMT2 antibody
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-SHMT2 antibody
Western Blot: Sample: Lane1: Human Lung Tissue; Lane2: Human 293T Cells; Lane3: Human Hela Cells; Lane4: Porcine Pancreas Tissue.
anti-SHMT2 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-SHMT2 antibody
Western Blot: Sample: Recombinant SHMT2, Human.
anti-SHMT2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SHMT2 antibody
DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.
anti-SHMT2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SHMT2 antibody
DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue
anti-SHMT2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SHMT2 antibody
DAB staining on IHC-P; Samples: Human Breast Cancer Tissue
anti-SHMT2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SHMT2 antibody
FITC staining on IHC-P Simple: Hela cells)
anti-SHMT2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SHMT2 antibody
DAB staining on IHC-P; Samples: Human Stomach Tissue
anti-SHMT2 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-SHMT2 antibody
Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: SHMT2 knockout 293T cell lysate; Predicted MW: 54kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human SHMT2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-SHMT2 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for SHMT2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
53,455 Da
NCBI Official Full Name
serine hydroxymethyltransferase, mitochondrial isoform 2
NCBI Official Synonym Full Names
serine hydroxymethyltransferase 2
NCBI Official Symbol
SHMT2  [Similar Products]
NCBI Official Synonym Symbols
GLYA; SHMT; HEL-S-51e
  [Similar Products]
NCBI Protein Information
serine hydroxymethyltransferase, mitochondrial
UniProt Protein Name
Serine hydroxymethyltransferase, mitochondrial
UniProt Synonym Protein Names
Glycine hydroxymethyltransferase; Serine methylase
UniProt Gene Name
SHMT2  [Similar Products]
UniProt Synonym Gene Names
NCBI Summary for SHMT2
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
UniProt Comments for SHMT2
SHMT2: Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.1; Energy Metabolism - methane; Methyltransferase; Mitochondrial; Other Amino Acids Metabolism - cyanoamino acid

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: cytoplasm; extracellular exosome; microtubule cytoskeleton; mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial matrix; mitochondrion; nucleus

Molecular Function: amino acid binding; chromatin binding; glycine hydroxymethyltransferase activity; identical protein binding; L-allo-threonine aldolase activity; protein binding; pyridoxal phosphate binding

Biological Process: folic acid metabolic process; glycine biosynthetic process from serine; glycine metabolic process; L-serine biosynthetic process; L-serine metabolic process; one-carbon metabolic process; positive regulation of cell proliferation; protein homotetramerization; protein tetramerization; tetrahydrofolate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated with anti-SHMT2 antibody
Diseases associated with anti-SHMT2 antibody
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