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anti-TBK1 antibody :: Rabbit anti-Human Serine/threonine-protein kinase TBK1 Polyclonal Antibody

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Catalog # MBS7004345
Unit / Price
Scan QR to view Datasheet
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

Serine/threonine-protein kinase TBK1, Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human Serine/threonine-protein kinase TBK1 polyclonal Antibody

Product Synonym Names
NF-kappa-B-activating kinase T2K TANK-binding kinase 1 TBK1 NAK
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
177700
3D Structure
ModBase 3D Structure for Q9UHD2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Immunogen
Recombinant human Serine/threonine-protein kinase TBK1 protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-33895 / sc-33896 / sc-33897 / sc-7221
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-TBK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TBK1 antibody
Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein.
Applications Tested/Suitable for anti-TBK1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TBK1 antibody
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200

Immunohistochemistry (IHC) of anti-TBK1 antibody
Immunohistochemistry of paraffin-embedded human liver tissue using MBS7004345 at dilution 1:100
anti-TBK1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-TBK1 antibody
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7004345 at dilution 1:100
anti-TBK1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-TBK1 antibody
Immunofluorescence staining of Hela cells cells with MBS7004345 at 1:333,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
anti-TBK1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for TBK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
83,642 Da[Similar Products]
NCBI Official Full Name
serine/threonine-protein kinase TBK1
NCBI Official Synonym Full Names
TANK-binding kinase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
NAK; T2K; FTDALS4
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase TBK1
UniProt Protein Name
Serine/threonine-protein kinase TBK1
UniProt Synonym Protein Names
NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
TBK1_HUMAN
NCBI Summary for TBK1
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. [provided by RefSeq, Oct 2010]
UniProt Comments for TBK1
TBK1: a protein kinase of the IKK family. Can mediate NFkB activation in response to certain growth factors. Forms a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: cytoplasm; cytosol; endosome membrane

Molecular Function: ATP binding; nucleic acid binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: defense response to Gram-positive bacterium; defense response to virus; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; interferon type I production; MyD88-independent toll-like receptor signaling pathway; negative regulation of interferon type I production; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interferon-alpha production; positive regulation of interferon-beta biosynthetic process; positive regulation of interferon-beta production; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to virus; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway; viral reproduction

Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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