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anti-SHC3 antibody :: Rabbit anti-Human SHC-Transforming Protein 3 Polyclonal Antibody

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Catalog # MBS2027238 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

SHC-Transforming Protein 3 (SHC3), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to SHC-Transforming Protein 3 (SHC3)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
D84361 mRNA
3D Structure
ModBase 3D Structure for Q92529
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
The antibody is a rabbit polyclonal antibody raised against SHC3. It has beenselected for its ability to recognize SHC3 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
SHC3 (Val188~Gln433)
Organism Species
Homo sapiens (Human)
Conjugate
No Conjugate
Immunogen
Recombinant SHC3 (Val188~Gln433) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2074955
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-SHC3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-SHC3 antibody
Applications Tested/Suitable for anti-SHC3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-SHC3 antibody
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-SHC3 antibody
Western Blot: Sample: Lane1: Porcine Brain Tissue; Lane2: Human HepG2 Cells.
anti-SHC3 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-SHC3 antibody
Western Blot: Sample: Recombinant SHC3, Human.
anti-SHC3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SHC3 antibody
DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.
anti-SHC3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SHC3 antibody
DAB staining on IHC-P; Samples: Human Liver Tissue
anti-SHC3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SHC3 antibody
DAB staining on IHC-P; Samples: Human Kidney Tissue
anti-SHC3 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-SHC3 antibody
Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: SHC3 knockout HepG2 cell lysate; Predicted MW: 64,51kd Observed MW: 51kd Primary Ab: 3ug/ml Rabbit Anti-Human SHC3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-SHC3 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for SHC3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
51,939 Da
NCBI Official Full Name
SHC-transforming protein 3
NCBI Official Synonym Full Names
SHC adaptor protein 3
NCBI Official Symbol
NCBI Official Synonym Symbols
RAI; NSHC; SHCC; N-Shc
  [Similar Products]
NCBI Protein Information
SHC-transforming protein 3
UniProt Protein Name
SHC-transforming protein 3
UniProt Synonym Protein Names
Neuronal Shc; N-Shc; Protein Rai; SHC-transforming protein C; Src homology 2 domain-containing-transforming protein C3; SH2 domain protein C3
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NSHC; SHCC; N-Shc; SH2 domain protein C3  [Similar Products]
UniProt Comments for SHC3
SHC3: an adapter protein containing an SH2 and a PID domain. Couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons and the regulation of NMDA receptor function in the hippocampus. May modulate hippocampal synaptic plasticity underlying learning and memory. Interacts with the Trk receptors in a phosphotyrosine-dependent manner. Once activated, binds to GRB2. Interacts with activated EGF receptors. Mainly expressed in brain. Hardly detectable in other tissues, except in pancreas. Highly expressed in the cerebral cortex, frontal and temporal lobes, occipital pole, hippocampus, caudate nucleus and amygdala. Expressed at low level in the cerebellum, medulla and spinal cord. A switch from Shc1 to Shc3 occurs during maturation of neuronal precursors to postmitotic neurons. Two alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q22.1

Cellular Component: cytosol; plasma membrane

Molecular Function: phosphotyrosine binding; protein binding; Ras guanyl-nucleotide exchange factor activity; receptor tyrosine kinase binding; signal transducer activity

Biological Process: axon guidance; central nervous system development; epidermal growth factor receptor signaling pathway; learning and/or memory; MAPK cascade; Ras protein signal transduction; regulation of molecular function; synaptic transmission, glutamatergic; transmembrane receptor protein tyrosine kinase signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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