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anti-SPAM1 antibody :: Rabbit anti-Human SPAM1 Polyclonal Antibody

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Catalog # MBS2520659
Unit / Price
  0.06 mL  /  $150 +1 FREE 8GB USB
  0.12 mL  /  $225 +1 FREE 8GB USB
  0.2 mL  /  $360 +1 FREE 8GB USB
Product Name

SPAM1, Polyclonal Antibody

Popular Item
Also Known As

SPAM1 Antibody

Product Synonym Names
HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
509
OMIM
600930
3D Structure
ModBase 3D Structure for P38567
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Affinity purification
Immunogen
Recombinant protein of human SPAM1
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SPAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SPAM1 antibody
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21. 3 and 7q31. 3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21. 3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Applications Tested/Suitable for anti-SPAM1 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-SPAM1 antibody
WB: 1:500-1:2000
IF: 1:50-1:200

Western Blot (WB) of anti-SPAM1 antibody
Western blot analysis of extracts of various cell lines, using SPAM1 antibody.
anti-SPAM1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-SPAM1 antibody
Immunofluorescence analysis of HeLa cell using SPAM1 antibody. Blue: DAPI for nuclear staining.
anti-SPAM1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for SPAM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
58kDa
NCBI Official Full Name
sperm adhesion molecule gene SPAM1
NCBI Official Synonym Full Names
sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding)
NCBI Official Symbol
SPAM1  [Similar Products]
NCBI Official Synonym Symbols
HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; HEL-S-96n
  [Similar Products]
NCBI Protein Information
hyaluronidase PH-20; epididymis secretory sperm binding protein Li 96n; hyal-PH20; hyaluronoglucosaminidase PH-20; sperm surface protein PH-20
UniProt Protein Name
Hyaluronidase PH-20
UniProt Synonym Protein Names
Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface protein PH-20
Protein Family
UniProt Gene Name
SPAM1  [Similar Products]
UniProt Synonym Gene Names
HYAL3; PH20; Hyal-PH20  [Similar Products]
UniProt Entry Name
HYALP_HUMAN
NCBI Summary for SPAM1
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
UniProt Comments for SPAM1
SPAM1: Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Belongs to the glycosyl hydrolase 56 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Membrane protein, GPI anchor; EC 3.2.1.35; Cell surface; Glycan Metabolism - glycosaminoglycan degradation

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: plasma membrane

Molecular Function: hyalurononglucosaminidase activity

Biological Process: sperm-egg recognition; fusion of sperm to egg plasma membrane; binding of sperm to zona pellucida; single fertilization; carbohydrate metabolic process; cell adhesion; multicellular organism reproduction
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Products associated withanti-SPAM1 antibody
 Reference Product  PubMed Publications
 HAS2 antibody  >2 publications with SPAM1 and HAS2
Organs/Tissues associated with anti-SPAM1 antibody
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