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anti-SQSTM1 antibody :: Rabbit anti-Human SQSTM1 Polyclonal Antibody

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Catalog # MBS9131527 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

SQSTM1, Polyclonal Antibody

Popular Item
Full Product Name

SQSTM1 Polyclonal Antibody

Product Synonym Names
A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
U41806 mRNA
3D Structure
ModBase 3D Structure for Q13501
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Affinity Purification
Immunogen
Recombinant protein of human SQSTM1
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-SQSTM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SQSTM1 antibody
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Product Categories/Family for anti-SQSTM1 antibody
Applications Tested/Suitable for anti-SQSTM1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-SQSTM1 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IP: 1:20 - 1:50

Western Blot (WB) of anti-SQSTM1 antibody
Western blot analysis of extracts of various cell lines, using SQSTM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
anti-SQSTM1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SQSTM1 antibody
Immunohistochemistry of paraffin-embedded human esophagus using SQSTM1 antibody at dilution of 1:100 (40x lens).
anti-SQSTM1 antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-SQSTM1 antibody
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug SQSTM1 antibody. Western blot was performed from the immunoprecipitate using SQSTM1 antibody at a dilition of 1:1000.
anti-SQSTM1 antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for SQSTM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48kDa
NCBI Official Full Name
Sequestosome-1
NCBI Official Synonym Full Names
sequestosome 1
NCBI Official Symbol
SQSTM1  [Similar Products]
NCBI Official Synonym Symbols
p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
  [Similar Products]
NCBI Protein Information
sequestosome-1
UniProt Protein Name
Sequestosome-1
UniProt Synonym Protein Names
EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62
Protein Family
UniProt Gene Name
SQSTM1  [Similar Products]
UniProt Synonym Gene Names
ORCA; OSIL; EBIAP; p60  [Similar Products]
NCBI Summary for SQSTM1
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
UniProt Comments for SQSTM1
SQSTM1: an adapter protein which binds ubiquitin, shuttling proteins to the proteasome. Co-localizes along with the E3 ubiquitin ligase, TRAF6, to aggregates from Alzheimer's disease brains but not in control brain. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Forms ternary complexes with PKCZ and Kv-beta2 or PKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Interacts with EBI3, LCK, RASA1, PKCZ , PKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MEK5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63- polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MEK5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Nuclear receptor co-regulator; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: autophagic vacuole; cytoplasm; cytosol; inclusion body; intracellular membrane-bound organelle; nucleoplasm; PML body

Molecular Function: identical protein binding; protein binding; protein kinase binding; protein kinase C binding; receptor tyrosine kinase binding; SH2 domain binding; ubiquitin binding; ubiquitin protein ligase binding

Biological Process: autophagy; endosome transport; macroautophagy; macromitophagy; negative regulation of apoptosis; positive regulation of apoptosis; positive regulation of transcription from RNA polymerase II promoter; protein localization; regulation of I-kappaB kinase/NF-kappaB cascade; response to stress; ubiquitin-dependent protein catabolic process

Disease: Frontotemporal Dementia And/or Amyotrophic Lateral Sclerosis 3; Myopathy, Distal, With Rimmed Vacuoles; Neurodegeneration With Ataxia, Dystonia, And Gaze Palsy, Childhood-onset; Paget Disease Of Bone
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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