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anti-TCRB antibody :: Rabbit anti-Human TCRB Polyclonal Antibody

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Catalog # MBS9205364
Unit / Price
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  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

TCRB, Polyclonal Antibody

Popular Item
Full Product Name

TCRB Antibody (Center)

Product Synonym Names
T-cell receptor beta chain V region CTL-L17; TCRB
Antibody/Peptide Pairs
TCRB peptide (MBS9218924) is used for blocking the activity of TCRB antibody (MBS9205364)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
67-94
3D Structure
ModBase 3D Structure for P04435
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB32261
Host
Rabbit
Species Reactivity
Human
Specificity
This TCRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-94 amino acids from the Central region of human TCRB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-TCRB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TCRB antibody
The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain.

T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.
Product Categories/Family for anti-TCRB antibody
Applications Tested/Suitable for anti-TCRB antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-TCRB antibody
WB~~1:1000

Western Blot (WB) of anti-TCRB antibody
All lanes : Anti-TCRB Antibody (Center)(MBS9205364) at 1:1000 dilution
Lane 1: Jurkat whole cell lysates
Lane 2: MOLT-4 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 15 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-TCRB antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-TCRB antibody
Anti-TCRB Antibody (Center)(MBS9205364) at 1:500 dilution + MDA-MB-231 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 15 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-TCRB antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-TCRB antibody
Anti-TCRB Antibody (Center) at 1:1000 dilution + human spleen lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size :15 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-TCRB antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-TCRB antibody
TCRB Antibody (Center) western blot analysis in MDA-MB231,HEK293,Ramos,NCI-H460 cell line lysates (35ug/lane).This demonstrates the TCRB antibody detected the TCRB protein (arrow).
anti-TCRB antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-TCRB antibody
Anti-TCRB Antibody (Center) at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 15 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
anti-TCRB antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TCRB. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
UniProt Primary Accession #
Molecular Weight
14999
NCBI Official Full Name
T-cell receptor beta chain V region CTL-L17
UniProt Protein Name
T-cell receptor beta chain V region CTL-L17
Protein Family
UniProt Gene Name
UniProt Entry Name
TVB2_HUMAN
UniProt Comments for TCRB
TCRB:

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 7q34
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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