• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-TET2 antibody :: Rabbit anti-Human TET2 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS151534
Unit / Price
Scan QR to view Datasheet
  0.1 mg  /  $375 +1 FREE 8GB USB
Western Blot (WB)
Product Name

TET2, Polyclonal Antibody

Full Product Name

TET2 Antibody

Product Synonym Names
TET2; MDS; KIAA1546; Nbla00191; Methylcytosine dioxygenase TET2; tet methylcytosine dioxygenase 2
Antibody/Peptide Pairs
TET2 peptide (MBS152115) is used for blocking the activity of TET2 antibody (MBS151534)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 614286
3D Structure
ModBase 3D Structure for Q6N021
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Specificity
At least two isoforms of TET2 are known to exist; this antibody will detect both isoforms.
Purity/Purification
TET2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
TET2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TET2.
Buffer
TET2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TET2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-TET2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TET2 antibody
TET2 Antibody: TET2, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. It is a candidate tumor suppressor gene reported to be mutated in approximately 14% of patients with JAK2V617F-positive myeloproliferative neoplasms, and can be mutated in other hematopoietic disorders such as myelodysplastic syndromes, acute myeloid leukemia, and chronic myelomonocytic leukemia. Analysis of the TET2 and JAK2 mutations in these neoplasms suggests that mutations in TET2 do not represent a predisposition for acquiring mutations in JAK2.
Applications Tested/Suitable for anti-TET2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-TET2 antibody
TET2 antibody can be used for detection of TET2 by Western blot at 1 mug/mL.

Western Blot (WB) of anti-TET2 antibody
Western blot analysis of TET2 in SK-N-SH cell lysate with TET2 antibody at 1 μg/mL.
anti-TET2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TET2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
133,481 Da
NCBI Official Full Name
methylcytosine dioxygenase TET2 isoform a
NCBI Official Synonym Full Names
tet methylcytosine dioxygenase 2
NCBI Official Symbol
NCBI Official Synonym Symbols
MDS; KIAA1546
  [Similar Products]
NCBI Protein Information
methylcytosine dioxygenase TET2; tet oncogene family member 2; probable methylcytosine dioxygenase TET2
UniProt Protein Name
Methylcytosine dioxygenase TET2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
KIAA1546  [Similar Products]
UniProt Entry Name
TET2_HUMAN
NCBI Summary for TET2
The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
UniProt Comments for TET2
TET2: Catalyzes the conversion of methylcytosine (5mC) to 5- hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. Defects in TET2 are a cause of polycythemia vera (PV). A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly. TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen. Defects in TET2 are a cause of myelodysplastic syndrome (MDS). A heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. Belongs to the TET family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.11.n2; Oxidoreductase; Tumor suppressor

Chromosomal Location of Human Ortholog: 4q24

Molecular Function: protein binding; DNA binding; zinc ion binding; ferrous iron binding

Biological Process: spleen development; protein amino acid O-linked glycosylation; hemoglobin metabolic process; cell cycle; regulation of gene expression, epigenetic; post-embryonic development; cytosine metabolic process; myeloid progenitor cell differentiation; 5-methylcytosine catabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation; kidney development

Disease: Myelodysplastic Syndrome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000