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anti-TFAM antibody :: Rabbit anti-Human TFAM Polyclonal Antibody

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Catalog # MBS7004771
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

TFAM, Polyclonal Antibody

Popular Item
Full Product Name

TFAM Antibody

Product Synonym Names
Transcription factor A; mitochondrial; mtTFA; Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2; TFAM; TCF6; TCF6L2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
600438
3D Structure
ModBase 3D Structure for Q00059
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Immunogen
Recombinant human Transcription factor A, mitochondrial protrin (43-246AA)
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30963 / sc-30962 / sc-19050 / sc-28200
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-TFAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TFAM antibody
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.
Applications Tested/Suitable for anti-TFAM antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TFAM antibody
Recommended dilution: WB:1:500-2000, IHC:1:20-1:200

Western Blot (WB) of anti-TFAM antibody
Western blot
All lanes: TFAM antibody at 8ug/ml
Lane 1:Jurkat whole cell lysate
Lane 2:MCF-7 whole cell lysate
Lane 3:A431 whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 30,26 kDa
Observed band size: 25 kDa

anti-TFAM antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-TFAM antibody
Immunohistochemistry of paraffin-embedded human testis using MBS7004771 at dilution 1:100
anti-TFAM antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for TFAM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
25,466 Da
NCBI Official Full Name
transcription factor A, mitochondrial isoform 2
NCBI Official Synonym Full Names
transcription factor A, mitochondrial
NCBI Official Symbol
NCBI Official Synonym Symbols
TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3
  [Similar Products]
NCBI Protein Information
transcription factor A, mitochondrial
UniProt Protein Name
Transcription factor A, mitochondrial
UniProt Synonym Protein Names
Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
TCF6; TCF6L2; mtTFA; MtTF1; TCF-6  [Similar Products]
UniProt Entry Name
TFAM_HUMAN
NCBI Summary for TFAM
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Comments for TFAM
TFAM: regulates mitochondrial transcription. Induced in humans by caloric restriction. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Activates transcription by binding immediately upstream of transcriptional start sites. Is able to unwind and bend DNA. Involved in cisplatin resistance. Interacts with TFB1M and TFB2M.

Protein type: DNA-binding; Transcription regulation

Chromosomal Location of Human Ortholog: 10q21

Cellular Component: cytosol; mitochondrial matrix; mitochondrion; nucleus

Molecular Function: chromatin binding; DNA bending activity; heat shock protein binding; protein binding; transcription factor activity

Biological Process: DNA-dependent DNA replication; gene expression; mitochondrial respiratory chain complex assembly; mitochondrion organization and biogenesis; organelle organization and biogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription from RNA polymerase I promoter; transcription from mitochondrial promoter; transcription from RNA polymerase II promoter; transcription initiation from mitochondrial promoter
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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