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anti-TIE2 antibody :: Rabbit anti-Human TIE2 Polyclonal Antibody

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Catalog # MBS9406636
Unit / Price
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Western Blot (WB)
Product Name

TIE2, Polyclonal Antibody

Popular Item
Also Known As

TIE2 (Phospho-Tyr992) Antibody

Product Synonym Names
Angiopoietin-1 receptor,EC=2.7.10.1 Tunica interna endothelial cell kinase Tyrosine-protein kinase receptor TEK Tyrosine-protein kinase receptor TIE-2 hTIE2 p140 TEK CD202b TEK TIE2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
1124
OMIM
600195
3D Structure
ModBase 3D Structure for Q02763
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
The Antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of tyrosine 992 (E-V-Y(p)-V-K) derived from Human TIE2.
Modification
Phospho-Tyr992
Target Name
TIE2
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-TIE2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TIE2 antibody
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.Schooley K.A., Baum P.R. Oncogene 8:663-670(1993)Dunham I. Nature 429:369-374(2004)Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Product Categories/Family for anti-TIE2 antibody
Applications Tested/Suitable for anti-TIE2 antibody
Western Blot (WB)
Application Notes for anti-TIE2 antibody
Western blotting: 1:500~1:1000

Western Blot (WB) of anti-TIE2 antibody
Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody.The lane on the right is treated with the antigen-specific peptide.
anti-TIE2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TIE2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
109,141 Da
NCBI Official Full Name
angiopoietin-1 receptor isoform 1
NCBI Official Synonym Full Names
TEK tyrosine kinase, endothelial
NCBI Official Symbol
NCBI Official Synonym Symbols
TIE2; VMCM; TIE-2; VMCM1; CD202B
  [Similar Products]
NCBI Protein Information
angiopoietin-1 receptor
UniProt Protein Name
Angiopoietin-1 receptor
UniProt Synonym Protein Names
Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; hTIE2; p140 TEK; CD_antigen: CD202b
UniProt Gene Name
UniProt Synonym Gene Names
TIE2; VMCM; VMCM1; hTIE2  [Similar Products]
UniProt Entry Name
TIE2_HUMAN
NCBI Summary for TIE2
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
UniProt Comments for TIE2
TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Membrane protein, integral; TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: microvillus; focal adhesion; cell surface; basolateral plasma membrane; integral to plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; stress fiber; basal plasma membrane; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; growth factor binding; transmembrane receptor protein tyrosine kinase activity; receptor activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; peptidyl-tyrosine phosphorylation; response to cAMP; protein amino acid autophosphorylation; cell-matrix adhesion; heart development; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; cell-cell signaling; positive regulation of focal adhesion formation; angiogenesis; endochondral ossification; Tie receptor signaling pathway; organ regeneration; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; negative regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; endothelial cell proliferation; negative regulation of inflammatory response; response to hypoxia; positive regulation of endothelial cell proliferation; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; regulation of vascular permeability; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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