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anti-TREM2 antibody :: Goat TREM2 Polyclonal Antibody

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Catalog # MBS420713
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

TREM2, Polyclonal Antibody

Also Known As

Goat anti-TREM2 Antibody

Product Synonym Names
TREM2; triggering receptor expressed on myeloid cells 2; TREM-2; Trem2a; Trem2b; Trem2c; OTTHUMP00000039771; triggering receptor expressed on myeloid cells 2a; TREM2 antibody; triggering receptor expressed on myeloid cells 2 antibody; TREM-2 antibody; Trem2a antibody; Trem2b antibody; Trem2c antibody; OTTHUMP00000039771 antibody; triggering receptor expressed on myeloid cells 2a antibody
Antibody/Peptide Pairs
TREM2 peptide (MBS426601) is used for blocking the activity of TREM2 antibody (MBS420713)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
230
Immunogen Sequence
HGQKPGTHPP SELD
OMIM
221770
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-HGQKPGTHPPSELD, from the C Terminus of the protein sequence according to NP_061838.1.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-TREM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TREM2 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-TREM2 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Spleen shows staining of macrophages. Recommended concentration, 2-3ug/ml.
Western Blot: Preliminary experiments gave bands at approx 80kDa and 30kDa in Human Brain (Cerebellum) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 25.4kDa according to NP_061838.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Immunohistochemistry (IHC) of anti-TREM2 antibody
(2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-TREM2 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for TREM2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,669 Da
NCBI Official Full Name
triggering receptor expressed on myeloid cells 2 isoform 1
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 2
NCBI Official Symbol
TREM2  [Similar Products]
NCBI Official Synonym Symbols
TREM-2; Trem2a; Trem2b; Trem2c
  [Similar Products]
NCBI Protein Information
triggering receptor expressed on myeloid cells 2
UniProt Protein Name
Triggering receptor expressed on myeloid cells 2
UniProt Synonym Protein Names
Triggering receptor expressed on monocytes 2
UniProt Gene Name
TREM2  [Similar Products]
UniProt Synonym Gene Names
TREM-2  [Similar Products]
UniProt Entry Name
TREM2_HUMAN
NCBI Summary for TREM2
This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
UniProt Comments for TREM2
TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular region; integral to membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: lipopolysaccharide binding; peptidoglycan binding; receptor activity

Biological Process: detection of lipopolysaccharide; detection of peptidoglycan; humoral immune response; innate immune response; phagocytosis, engulfment; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of calcium-mediated signaling; positive regulation of peptidyl-tyrosine phosphorylation; regulation of immune response

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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