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anti-TRIM33 antibody :: Rabbit anti-Human TRIM33 Polyclonal Antibody

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Catalog # MBS9213223
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Western Blot (WB)
Product Name

TRIM33, Polyclonal Antibody

Popular Item
Full Product Name

TRIM33 Antibody (C-term)

Product Synonym Names
E3 ubiquitin-protein ligase TRIM33; 632-; Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33; TRIM33; KIAA1113; RFG7; TIF1G
Antibody/Peptide Pairs
TRIM33 peptide (MBS9220071) is used for blocking the activity of TRIM33 antibody (MBS9213223)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
820-849
OMIM
188550
3D Structure
ModBase 3D Structure for Q9UPN9
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Specificity
This TRIM33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-849 amino acids from the C-terminal region of human TRIM33.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Cellular Location
Nucleus. Note=In discrete nuclear dots resembling nuclear bodies.
Tissue Location
Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-TRIM33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TRIM33 antibody
The protein encoded by this gene is thought to be a
transcriptional corepressor. However, molecules that interact with
this protein have not yet been identified. The protein is a member
of the tripartite motif family. This motif includes three
zinc-binding domains, a RING, a B-box type 1 and a B-box type 2,
and a coiled-coil region. Three alternatively spliced transcript
variants for this gene have been described, however, the
full-length nature of one variant has not been determined.

Function: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade).
Product Categories/Family for anti-TRIM33 antibody
Applications Tested/Suitable for anti-TRIM33 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-TRIM33 antibody
WB~~1:1000

Western Blot (WB) of anti-TRIM33 antibody
TRIM33 Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TRIM33 antibody detected the TRIM33 protein (arrow).
anti-TRIM33 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TRIM33. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
122533
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM33 isoform alpha
NCBI Official Synonym Full Names
tripartite motif containing 33
NCBI Official Symbol
TRIM33  [Similar Products]
NCBI Official Synonym Symbols
ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
  [Similar Products]
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM33
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM33
UniProt Synonym Protein Names
Ectodermin homolog; RET-fused gene 7 protein; Protein Rfg7; Transcription intermediary factor 1-gamma; TIF1-gamma; Tripartite motif-containing protein 33
UniProt Gene Name
TRIM33  [Similar Products]
UniProt Synonym Gene Names
KIAA1113; RFG7; TIF1G; Protein Rfg7; TIF1-gamma  [Similar Products]
UniProt Entry Name
TRI33_HUMAN
NCBI Summary for TRIM33
The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]
UniProt Comments for TRIM33
TIF1G: an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described.

Protein type: EC 6.3.2.19; Ligase; EC 6.3.2.-; Protein kinase, atypical; Kinase, protein; Transcription, coactivator/corepressor; Ubiquitin ligase; Ubiquitin conjugating system; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway

Disease: Thyroid Carcinoma, Papillary
Product References and Citations for anti-TRIM33 antibody
Bai, X., et al. Cell 142(1):133-143(2010)
Howard, P.W., et al. Biochem. Biophys. Res. Commun. 396(3):674-678(2010)
Vincent, D.F., et al. PLoS Genet. 5 (7), E1000575 (2009) :
Dupont, S., et al. Cell 136(1):123-135(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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