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anti-TRIM5 antibody :: Rabbit anti-Human TRIM5 Polyclonal Antibody

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Catalog # MBS240305
Unit / Price
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  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

TRIM5, Polyclonal Antibody

Popular Item
Full Product Name

Rabbit Polyclonal to Human TRIM5

Product Synonym Names
Anti-TRIM5 Antibody IHC-plus; TRIM5; RNF88; RING finger protein 88; Tripartite motif containing 5; TRIM5alpha; Tripartite motif protein TRIM5; Tripartite motif-containing 5; Human TRIM5
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
608487
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
synthetic peptide corresponding to amino acids near the mid-region of human TRIM5a
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human TRIM5.
Immunogen Type
Synthetic peptide
Immunogen
TRIM5 antibody was raised against synthetic peptide from human TRIM5.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Other Notes
Small volumes of anti-TRIM5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TRIM5 antibody
TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the a variant which also contains a carboxy-terminal B30.2 (SPRY) domain (1).
Product Categories/Family for anti-TRIM5 antibody
Applications Tested/Suitable for anti-TRIM5 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-TRIM5 antibody
IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...

Immunohistochemistry (IHC) of anti-TRIM5 antibody
Anti-TRIM5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
anti-TRIM5 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-TRIM5 antibody
Immunofluorescence of TRIM5 alpha in Human Stomach cells with TRIM5 alpha antibody at 10 ug/ml.
anti-TRIM5 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-TRIM5 antibody
Western blot of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 ug /ml.
anti-TRIM5 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TRIM5. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
29,677 Da
NCBI Official Full Name
Homo sapiens tripartite motif containing 5 (TRIM5), RefSeqGene on chromosome 11
NCBI Official Synonym Full Names
tripartite motif containing 5
NCBI Official Symbol
TRIM5  [Similar Products]
NCBI Official Synonym Symbols
RNF88; TRIM5alpha
  [Similar Products]
NCBI Protein Information
tripartite motif-containing protein 5; ring finger protein 88; tripartite motif protein TRIM5; tripartite motif containing 5 transcript variant iota; tripartite motif containing 5 transcript variant kappa
UniProt Protein Name
Tripartite motif-containing protein 5
UniProt Synonym Protein Names
RING finger protein 88
UniProt Gene Name
TRIM5  [Similar Products]
UniProt Synonym Gene Names
RNF88  [Similar Products]
UniProt Entry Name
TRIM5_HUMAN
NCBI Summary for TRIM5
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009]
UniProt Comments for TRIM5
TRIM5: Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; zinc ion binding; ubiquitin-protein ligase activity; pattern recognition receptor activity; ligase activity

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of MAPKKK cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of transcription factor activity; pattern recognition receptor signaling pathway; defense response to virus; activation of innate immune response; regulation of lipopolysaccharide-mediated signaling pathway; activation of NF-kappaB transcription factor
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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