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anti-Lck antibody :: Rabbit anti-Human Tyrosine-protein kinase Lck Polyclonal Antibody

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Catalog # MBS1498983
Unit / Price
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  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

Tyrosine-protein kinase Lck, Polyclonal Antibody

Popular Item
Full Product Name

Rabbit anti-human Tyrosine-protein kinase Lck polyclonal Antibody

Product Synonym Names
Leukocyte C-terminal Src kinase; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK; LCK
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
153390
3D Structure
ModBase 3D Structure for P06239
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human Tyrosine-protein kinase Lck protein(1-539aa)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-32014 / sc-13 / sc-28882
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Lck antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Lck antibody
Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
Applications Tested/Suitable for anti-Lck antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunohistochemistry (IHC) of anti-Lck antibody
Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS1498983 at dilution of 1:100
anti-Lck antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-Lck antibody
Immunohistochemistry of paraffin-embedded human lymph node tissue using MBS1498983 at dilution of 1:100
anti-Lck antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-Lck antibody
Immunofluorescent analysis of Hela cells using MBS1498983 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-Lck antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-Lck antibody
Immunoprecipitating LCK in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1ug)instead of MBS1498983 in Jurkat whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS1498983 (8ug)+ Jurkat whole cell lysate (500ug)
Lane 3: Jurkat whole cell lysate (10ug)

anti-Lck antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for Lck. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
61,190 Da
NCBI Official Full Name
tyrosine-protein kinase Lck
NCBI Official Synonym Full Names
LCK proto-oncogene, Src family tyrosine kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
LSK; YT16; IMD22; p56lck; pp58lck
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NCBI Protein Information
tyrosine-protein kinase Lck
UniProt Protein Name
Tyrosine-protein kinase Lck
UniProt Synonym Protein Names
Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
LCK_HUMAN
NCBI Summary for Lck
This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for Lck
Lck: a tyrosine kinase of the Src family that is crucial to antigen-receptor signaling in lymphocytes. plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of Sam68 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Overexpression in mice leads to thymic tumors. Aberrant expression is seen in T cell leukemias and colon cancer. The leukemic translocation t(1;7)(p34;q34) has breakpoints at the T cell receptor gene and close to the Lck promoters, can cause increased Lck expression, and in one case, point mutations. A mutated Lck has also been seen in a cell line. One patient with aberrant Lck splicing suffered from SCID-like T cell deficiency. Inhibitor: BMS-279700. Three alternatively spliced isoforms of the human proteinhave been described.

Protein type: Protein kinase, TK; Oncoprotein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: cytosol; extrinsic to internal side of plasma membrane; immunological synapse; intercellular junction; lipid raft; pericentriolar material; plasma membrane

Molecular Function: ATP binding; ATPase binding; CD4 receptor binding; CD8 receptor binding; glycoprotein binding; identical protein binding; non-membrane spanning protein tyrosine kinase activity; phosphoinositide 3-kinase binding; protein binding; protein C-terminus binding; protein kinase binding; protein phosphatase binding; protein serine/threonine phosphatase activity; protein-tyrosine kinase activity; SH2 domain binding

Biological Process: B cell receptor signaling pathway; blood coagulation; caspase activation; cellular zinc ion homeostasis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; hemopoiesis; innate immune response; leukocyte migration; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; platelet activation; positive regulation of gamma-delta T cell differentiation; positive regulation of T cell activation; positive regulation of T cell receptor signaling pathway; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid dephosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of defense response to virus by virus; regulation of lymphocyte activation; regulation of regulatory T cell differentiation; release of sequestered calcium ion into cytosol; response to drug; T cell costimulation; T cell differentiation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway; viral reproduction

Disease: Immunodeficiency 22
Precautions
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Disclaimer
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