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anti-ATP6V1E1 antibody :: Rabbit anti-Human V-type proton ATPase subunit E 1 Polyclonal Antibody

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Catalog # MBS715499
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

V-type proton ATPase subunit E 1 (ATP6V1E1), Polyclonal Antibody

Popular Item
Also Known As

Rabbit anti-human V-type proton ATPase subunit E 1 polyclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
204
OMIM
108746
3D Structure
ModBase 3D Structure for P36543
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation Purified
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant human V-type proton ATPase subunit E 1 protein
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21218 / sc-20946
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-ATP6V1E1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ATP6V1E1 antibody
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Applications Tested/Suitable for anti-ATP6V1E1 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

Western Blot (WB) of anti-ATP6V1E1 antibody
Western blot
V-type proton ATPase subunit E 1 Antibody at 2ug/ml+293T whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 27,24,23 kDa
Observed band size: 36 kDa

anti-ATP6V1E1 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-ATP6V1E1 antibody
Immunofluorescent analysis of Hela cells using MBS715499 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-ATP6V1E1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ATP6V1E1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
22,706 Da[Similar Products]
NCBI Official Full Name
V-type proton ATPase subunit E 1 isoform b
NCBI Official Synonym Full Names
ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
NCBI Official Symbol
ATP6V1E1  [Similar Products]
NCBI Official Synonym Symbols
P31; Vma4; ATP6E; ATP6E2; ATP6V1E
  [Similar Products]
NCBI Protein Information
V-type proton ATPase subunit E 1
UniProt Protein Name
V-type proton ATPase subunit E 1
UniProt Synonym Protein Names
V-ATPase 31 kDa subunit; p31; Vacuolar proton pump subunit E 1
Protein Family
UniProt Gene Name
ATP6V1E1  [Similar Products]
UniProt Synonym Gene Names
ATP6E; ATP6E2; V-ATPase subunit E 1; p31  [Similar Products]
UniProt Entry Name
VATE1_HUMAN
NCBI Summary for ATP6V1E1
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq, Jul 2008]
UniProt Comments for ATP6V1E1
ATP6V1E1: Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the V-ATPase E subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Mitochondrial; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: apical plasma membrane; cytosol; endosome; lysosomal membrane; microvillus; mitochondrion; proton-transporting two-sector ATPase complex

Molecular Function: ATPase binding; hydrogen ion transporting ATPase activity, rotational mechanism; hydrogen-exporting ATPase activity, phosphorylative mechanism; protein binding

Biological Process: ATP hydrolysis coupled proton transport; cell redox homeostasis; cellular iron ion homeostasis; insulin receptor signaling pathway; metabolic process; proton transport; transferrin transport; transmembrane transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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