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anti-VEGF165 antibody :: Rabbit anti-Human VEGF165 Polyclonal Antibody

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Catalog # MBS8500092
Unit / Price
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  0.1 mg  /  $290 +1 FREE 8GB USB
Western Blot (WB)
Product Name

VEGF165, Polyclonal Antibody

Full Product Name

VEGF165 Polyclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
192240
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
Purified by protein A column
Form/Format
Each vial at least contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of 0.01 M PBS, PH7.4, 1 mg/ml BSA, 20 % glycerol, 0.01 % (w/v) NaN3
Immunogen
Recombinant human VEGF165, 27~191 aa of VEGF
Preparation and Storage
Store at-20 degree C upon arrival for one year. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-VEGF165 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-VEGF165 antibody
Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Six forms of human VEGF mRNA encoding VEGF proteins of 121, 145, 165, 183, 189, and 206 amino acids are produced from a single gene as a result of alternative splicing. VEGF165 appears to be the major gene product found in human tissue and is the most effective angiogenic factor in the VEGF family.
Applications Tested/Suitable for anti-VEGF165 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-VEGF165 antibody
WB: 1:1000-5000

Western Blot (WB) of anti-VEGF165 antibody
WB Assay 1st antibody: E14-05, dilution: 1:5000 2nd antibody: Goat antibody against Rabbit IgG conjugated with HRP, dilution: 1:5000, visualized by DAB Protein Molecular Wt.: 21 kDa M: Pre-stained protein marker 1: Recombinant human VEGF165
anti-VEGF165 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for VEGF165. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,406 Da
NCBI Official Full Name
vascular endothelial growth factor isoform VEGF165
NCBI Official Synonym Full Names
vascular endothelial growth factor A
NCBI Official Symbol
VEGFA  [Similar Products]
NCBI Official Synonym Symbols
VPF; VEGF; MVCD1
  [Similar Products]
NCBI Protein Information
vascular endothelial growth factor A
UniProt Protein Name
Vascular endothelial growth factor A
UniProt Synonym Protein Names
Vascular permeability factor; VPF
UniProt Gene Name
VEGFA  [Similar Products]
UniProt Synonym Gene Names
VEGF; VEGF-A; VPF  [Similar Products]
NCBI Summary for VEGF165
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2015]
UniProt Comments for VEGF165
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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