• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-VPS4B antibody :: Rabbit anti-Human VPS4B Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS7052497
Unit / Price
  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

VPS4B, Polyclonal Antibody

Popular Item
Full Product Name

VPS4B Antibody

Product Synonym Names
Vacuolar protein sorting-associated protein 4B; Cell migration-inducing gene 1 protein; Suppressor of K(+) transport growth defect 1; Protein SKD1; VPS4B; SKD1; VPS42; MIG1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF195514 mRNA
3D Structure
ModBase 3D Structure for O75351
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human Vacuolar protein sorting-associated protein 4B protein (1-117AA)
Conjugation
Non-conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-VPS4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-VPS4B antibody
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Applications Tested/Suitable for anti-VPS4B antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-VPS4B antibody
IHC: 1:20-1:200
IF: 1:50-1:200

Immunohistochemistry (IHC) of anti-VPS4B antibody
Immunohistochemistry of paraffin-embedded human small intestine tissue using MBS7052497 at dilution of 1:100
anti-VPS4B antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-VPS4B antibody
Immunofluorescent analysis of MCF-7 cells using MBS7052497 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-VPS4B antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-VPS4B antibody
Western Blot
Positive WB detected in: Mouse heart tissue,Mouse brain tissue
All lanes: VPS4B antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50 KDa
Observed band size: 50 KDa

anti-VPS4B antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for VPS4B. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,302 Da
NCBI Official Full Name
vacuolar protein sorting-associated protein 4B
NCBI Official Synonym Full Names
vacuolar protein sorting 4 homolog B
NCBI Official Symbol
VPS4B  [Similar Products]
NCBI Official Synonym Symbols
MIG1; SKD1; SKD1B; VPS4-2
  [Similar Products]
NCBI Protein Information
vacuolar protein sorting-associated protein 4B
UniProt Protein Name
Vacuolar protein sorting-associated protein 4B
UniProt Synonym Protein Names
Cell migration-inducing gene 1 protein; Suppressor of K(+) transport growth defect 1; Protein SKD1
UniProt Gene Name
VPS4B  [Similar Products]
UniProt Synonym Gene Names
SKD1; VPS42; Protein SKD1  [Similar Products]
NCBI Summary for VPS4B
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]
UniProt Comments for VPS4B
VPS4B: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Belongs to the AAA ATPase family.

Protein type: EC 3.6.4.6; Vesicle

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: centrosome; cytoplasm; cytosol; endosome; endosome membrane; nucleus; spindle pole

Molecular Function: ATPase activity; ATPase activity, coupled; identical protein binding; microtubule-severing ATPase activity; protein binding; protein C-terminus binding; protein homodimerization activity

Biological Process: cell separation during cytokinesis; cytoplasmic microtubule organization and biogenesis; endosome to lysosome transport via multivesicular body sorting pathway; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis; positive regulation of viral reproduction; protein depolymerization; regulation of viral reproduction; release of virus from host; response to lipid; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; vacuole organization and biogenesis; viral infectious cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000