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anti-XAF1 antibody :: Rabbit anti-Human XAF1 Polyclonal Antibody

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Catalog # MBS241350
Unit / Price
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  0.05 mg  /  $530 +1 FREE 8GB USB
Product Name

XAF1, Polyclonal Antibody

Full Product Name

Rabbit Polyclonal to Human XAF1

Product Synonym Names
Anti-XAF1 Antibody IHC-plus; XAF1; BIRC4 binding protein; BIRC4-binding protein; BIRC4BP; HSXIAPAF1; XIAP-associated factor 1; XIAPAF1; XIAP associated factor 1; Human XAF1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
606717
3D Structure
ModBase 3D Structure for Q6GPH4
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Specificity
synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide.
Concentration
1 mg/ml (lot specific)
Target Species
Human
Immunogen Description
Synthetic peptide from human XAF1.
Immunogen Type
Synthetic peptide
Immunogen
XAF1 antibody was raised against synthetic peptide from human XAF1.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.
Other Notes
Small volumes of anti-XAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-XAF1 antibody
XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines.
Product Categories/Family for anti-XAF1 antibody
Applications Tested/Suitable for anti-XAF1 antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes for anti-XAF1 antibody
IHC-P (10 ug/ml), WB (1 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...

Immunohistochemistry (IHC) of anti-XAF1 antibody
Anti-XAF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-XAF1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-XAF1 antibody
Immunofluorescence of XAF-1 in Human Spleen cells with XAF-1 antibody at 20 ug/ml.
anti-XAF1 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-XAF1 antibody
Western blot of XAF-1 in human spleen lysate with XAF-1 antibody (IN) at 1 (lane A), 2 (lane B), and 4 (lane C) ug/ml, respectively.
anti-XAF1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for XAF1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
34,543 Da
NCBI Official Full Name
XIAP-associated factor 1 isoform 1
NCBI Official Synonym Full Names
XIAP associated factor 1
NCBI Official Symbol
NCBI Official Synonym Symbols
BIRC4BP; XIAPAF1; HSXIAPAF1
  [Similar Products]
NCBI Protein Information
XIAP-associated factor 1; BIRC4 binding protein; BIRC4-binding protein
UniProt Protein Name
XIAP-associated factor 1
UniProt Synonym Protein Names
BIRC4-binding protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
BIRC4BP; XIAPAF1  [Similar Products]
UniProt Entry Name
XAF1_HUMAN
NCBI Summary for XAF1
This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
UniProt Comments for XAF1
BIRC4BP: Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: mitochondrion; cytosol; nucleus

Molecular Function: zinc ion binding

Biological Process: apoptosis; cytokine and chemokine mediated signaling pathway; negative regulation of protein complex assembly
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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