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anti-AATF antibody :: Rabbit AATF Polyclonal Antibody

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Catalog # MBS821781
Unit / Price
  0.03 mL  /  $155 +1 FREE 8GB USB
  0.1 mL  /  $220 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

AATF, Polyclonal Antibody

Also Known As

Anti-AATF Antibody

Product Synonym Names
CHE1; DED; Protein AATF; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1
Antibody/Peptide Pairs
AATF peptide (MBS822469) is used for blocking the activity of AATF antibody (MBS821781)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
560
OMIM
608463
3D Structure
ModBase 3D Structure for Q9NY61
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Specificity
Recognizes endogenous levels of AATF protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human AATF. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-AATF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-AATF antibody
Rabbit polyclonal antibody to AATF
Applications Tested/Suitable for anti-AATF antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-AATF antibody
WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100)

Western Blot (WB) of anti-AATF antibody
anti-AATF antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AATF antibody
Immunohistochemical analysis of AATF staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-AATF antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-AATF antibody
Immunofluorescent analysis of AATF staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-AATF antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
NCBI/Uniprot data below describe general gene information for AATF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
63,133 Da
NCBI Official Full Name
protein AATF
NCBI Official Synonym Full Names
apoptosis antagonizing transcription factor
NCBI Official Symbol
NCBI Official Synonym Symbols
DED; BFR2; CHE1; CHE-1
  [Similar Products]
NCBI Protein Information
protein AATF; rb-binding protein Che-1; apoptosis-antagonizing transcription factor
UniProt Protein Name
Protein AATF
UniProt Synonym Protein Names
Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
CHE1; DED  [Similar Products]
UniProt Entry Name
AATF_HUMAN
NCBI Summary for AATF
The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]
UniProt Comments for AATF
AATF: May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. Belongs to the AATF family.

Protein type: Nucleolus; Transcription factor; RNA-binding

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: Golgi apparatus; centrosome; focal adhesion; cytoplasm; nucleolus; nucleus

Molecular Function: leucine zipper domain binding; protein binding; tau protein binding; transcription factor activity

Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; negative regulation of amyloid precursor protein biosynthetic process; regulation of mitotic cell cycle; ribosome biogenesis and assembly; positive regulation of transcription from RNA polymerase II promoter; negative regulation of superoxide release; cell adhesion; response to DNA damage stimulus; embryonic cleavage; negative regulation of apoptosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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