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anti-ACADM antibody :: Rabbit ACADM Polyclonal Antibody

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Catalog # MBS9211145
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

ACADM, Polyclonal Antibody

Also Known As

ACADM Antibody (Center)

Product Synonym Names
Medium-chain specific acyl-CoA dehydrogenase; mitochondrial; MCAD; ACADM
Antibody/Peptide Pairs
ACADM peptide (MBS9226362) is used for blocking the activity of ACADM antibody (MBS9211145)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
189-217
Immunogen Sequence Length
425
OMIM
201450
3D Structure
ModBase 3D Structure for P11310
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Specificity
This ACADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the Central region of human ACADM.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ACADM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACADM antibody
ACADM is the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway.
Applications Tested/Suitable for anti-ACADM antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-ACADM antibody
WB~~1:1000

Western Blot (WB) of anti-ACADM antibody
Western blot analysis of ACADM Antibody (Center) in mouse heart tissue lysates (35ug/lane). ACADM (arrow) was detected using the purified Pab.
anti-ACADM antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ACADM antibody
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-ACADM antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-ACADM antibody
Flow cytometric analysis of widr cells using ACADM Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-ACADM antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunofluorescence (IF) of anti-ACADM antibody
Confocal immunofluorescent analysis of ACADM Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
anti-ACADM antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ACADM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Human, mouse (Predicted Reactivity: Monkey)NP_001120800.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46588
NCBI Official Full Name
medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase, C-4 to C-12 straight chain
NCBI Official Symbol
ACADM  [Similar Products]
NCBI Official Synonym Symbols
MCAD; ACAD1; MCADH
  [Similar Products]
NCBI Protein Information
medium-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Protein Name
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Gene Name
ACADM  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ACADM_HUMAN
NCBI Summary for ACADM
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ACADM
ACADM: This enzyme is specific for acyl chain lengths of 4 to 16. Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD). It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. Belongs to the acyl-CoA dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Other Amino Acids Metabolism - beta-alanine; Mitochondrial; Oxidoreductase; Carbohydrate Metabolism - propanoate; EC 1.3.8.7; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: mitochondrion; mitochondrial matrix; axon; nucleus

Molecular Function: identical protein binding; acyl-CoA dehydrogenase activity; FAD binding

Biological Process: carnitine metabolic process, CoA-linked; fatty acid beta-oxidation; medium-chain fatty acid catabolic process; cellular lipid metabolic process; medium-chain fatty acid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; carnitine biosynthetic process

Disease: Acyl-coa Dehydrogenase, Medium-chain, Deficiency Of
Product References and Citations for anti-ACADM antibody
Ferreira,A.C., et.al., Genet. Mol. Res. 8 (2), 487-493 (2009)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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