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anti-ACAT1 antibody :: Rabbit ACAT1 Polyclonal Antibody

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Catalog # MBS9206604
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

ACAT1, Polyclonal Antibody

Also Known As

ACAT1 Antibody (C-term)

Product Synonym Names
Acetyl-CoA acetyltransferase; mitochondrial; Acetoacetyl-CoA thiolase; T2; ACAT1; ACAT; MAT
Antibody/Peptide Pairs
ACAT1 peptide (MBS9226999) is used for blocking the activity of ACAT1 antibody (MBS9206604)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
296-329
Immunogen Sequence Length
427
OMIM
203750
3D Structure
ModBase 3D Structure for P24752
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Specificity
This ACAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 296-329 amino acids from the C-terminal region of human ACAT1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (lot specific)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ACAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ACAT1 antibody
ACAT1 is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Applications Tested/Suitable for anti-ACAT1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes for anti-ACAT1 antibody
WB~~1:1000

Western Blot (WB) of anti-ACAT1 antibody
All lanes : Anti-ACAT1 Antibody (C-term) at 1:2000 dilution
Lane 1: SW620 whole cell lysates
Lane 2: mouse liver lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 45. 2 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-ACAT1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACAT1 antibody
All lanes : Anti-ACAT1 Antibody (C-term) at 1:1000 dilution
Lane 1: SK-BR-3 whole cell lysates
Lane 2: HepG2 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 45. 2 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-ACAT1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACAT1 antibody
Western blot analysis of anti-ACAT1 Pab in SK-BR-3 cell line lysates (35ug/lane).ACAT1 (arrow) was detected using the purified Pab.
anti-ACAT1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ACAT1 antibody
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ACAT1 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-ACAT1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ACAT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45200
NCBI Official Full Name
acetyl-CoA acetyltransferase, mitochondrial
NCBI Official Synonym Full Names
acetyl-CoA acetyltransferase 1
NCBI Official Symbol
ACAT1  [Similar Products]
NCBI Official Synonym Symbols
T2; MAT; ACAT; THIL
  [Similar Products]
NCBI Protein Information
acetyl-CoA acetyltransferase, mitochondrial
UniProt Protein Name
Acetyl-CoA acetyltransferase, mitochondrial
UniProt Synonym Protein Names
Acetoacetyl-CoA thiolase; T2
UniProt Gene Name
ACAT1  [Similar Products]
UniProt Synonym Gene Names
ACAT; MAT  [Similar Products]
UniProt Entry Name
THIL_HUMAN
NCBI Summary for ACAT1
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
UniProt Comments for ACAT1
ACAT1: Plays a major role in ketone body metabolism. Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD); also known as alpha- methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3- hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. Belongs to the thiolase family.

Protein type: Carbohydrate Metabolism - butanoate; Lipid Metabolism - fatty acid; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Acetyltransferase; Amino Acid Metabolism - tryptophan; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Amino Acid Metabolism - lysine degradation; Carbohydrate Metabolism - propanoate; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; EC 2.3.1.9; Mitochondrial

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: protein homodimerization activity; enzyme binding; acetyl-CoA C-acetyltransferase activity; metal ion binding; coenzyme binding

Biological Process: response to starvation; ketone body catabolic process; response to hormone stimulus; ketone body biosynthetic process; ketone body metabolic process; branched chain family amino acid catabolic process; cellular lipid metabolic process; brain development; liver development; response to organic cyclic substance; protein homooligomerization

Disease: Alpha-methylacetoacetic Aciduria
Product References and Citations for anti-ACAT1 antibody
Locke,J.A.,Prostate 68 (1), 20-33 (2008)
Guo,Z.Y.,Biochemistry 46 (35), 10063-10071 (2007)
Haapalainen,A.M.,Biochemistry 46 (14), 4305-4321 (2007)

Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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