• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-HRI antibody :: Rabbit HRI Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS2538144
Unit / Price
  0.02 mL  /  $110 +1 FREE 8GB USB
  0.06 mL  /  $150 +1 FREE 8GB USB
  0.12 mL  /  $225 +1 FREE 8GB USB
  0.2 mL  /  $360 +1 FREE 8GB USB
Western Blot (WB)
Product Name

HRI, Polyclonal Antibody

Popular Item
Also Known As

EIF2AK1 Polyclonal Antibody

Product Synonym Names
EIF2AK1; HRI; KIAA1369; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS2538144 Technical Datasheet
3D Structure
ModBase 3D Structure for Q9BQI3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Monkey
Purity/Purification
Affinity Purification
Immunogen
Synthesized peptide derived from the C-terminal region of human HRI.
Buffer
PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Calculated MW
71kDa
Observed MW
71kDa
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-HRI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-HRI antibody
Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser-48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions.
Applications Tested/Suitable for anti-HRI antibody
WB, IHC-p, ELISA
Application Notes for anti-HRI antibody
WB: 1:500-1:2000
IHC: 1:100-1:300
ELISA: 1:4000

Western Blot (WB) of anti-HRI antibody
Western Blot analysis of A549 cells with EIF2AK1 Polyclonal Antibody.
anti-HRI antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for HRI. It may not necessarily be applicable to this product.
UniProt Primary Accession #
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000